Exploration on the Intervention Mechanism of Yiqi Tongbi Capsules in Ischemic Heart Failure Based on Network Pharmacology,Molecular Docking and Experimental Verification
10.19879/j.cnki.1005-5304.202410358
- VernacularTitle:基于网络药理学、分子对接及实验验证探讨益气通痹胶囊对缺血性心力衰竭干预机制
- Author:
Zhongzheng LI
1
;
Bin LI
1
;
Wenhui GUO
1
;
Jinjian GUO
1
Author Information
1. 福建中医药大学附属第二人民医院,福建 福州 350003
- Publication Type:Journal Article
- Keywords:
Yiqi Tongbi Capsules;
ischemic heart failure;
network pharmacology;
molecular docking;
autophagy
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(4):42-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Yiqi Tongbi Capsules in the treatment of ischemic heart failure(IHF)using network pharmacological method and molecular docking technique;To verify through in vivo experiments.Methods The effective components and corresponding target of Yiqi Tongbi Capsules were screened using TCMSP and BATMAN-TCM databases.The targets related to IHF disease were searched using GeneCards,OMIM and TTD databases.The common targets between the drug active components and IHF disease-related targets were obtained.A Chinese materia medica-active component-common target network was constructed to select the key targets.The common targets were input into the STRING database to obtain PPI network relationships,and the core targets were selected.The GO function and KEGG pathway enrichment analyses were performed on the common targets using the Metascape platform.The key active components and core targets were verified by molecular docking using the AutoDock 4.2.6 software.A mouse IHF model was established by ligating the left anterior descending branch of the coronary artery surgically,and the mice were divided into sham-operation group,model group,captopil group(3.25 mg/kg),Yiqi Tongbi Capsules low-,medium-,and high-dosage groups(2.34,4.68,9.36 g/kg).The heart function of mice was detected by Doppler ultrasound imaging system for 6 weeks after continuous administration;the mRNA expressions of LC3B,Beclin1,p62,EGFR and STAT3 were detected by RT-qPCR in mouse myocardial tissue.Results A total of 186 potential target genes for the therapeutic effects of Yiqi Tongbi Capsules on IHF were screened out;the top 5 key active components were quercetin,β-sitosterol,lutein,stigmasterol and baicalein;the core targets included AKT1,STAT3,HIF1A,BCL2 and EGFR,etc.The GO function and KEGG enrichment analysis showed that the core targets mainly participated in the processes of active oxygen metabolism,cytokine receptor binding,and involving the pathways of lipid and atherosclerosis,AGE-RAGE signaling pathway and IL-17 signaling pathway.The key active components and core target genes had good binding affinity.Yiqi Tongbi Capsules significantly improved the heart function of IHF mice;the RT-qPCR results showed that the EGFR/STAT3 signaling pathway and autophagy process were activated in the myocardial tissue of the model group mice,but the degree was not enough to improve IHF.However,Yiqi Tongbi Capsules could significantly up-regulate the expression levels of LC3B,Beclin1 mRNA in the myocardial tissue of mice,and significantly down-regulate the expression of p62,EGFR and STAT3 mRNA.Conclusion Yiqi Tongbi Capsules may playing a role in the treatment of IHF by regulating quercetin,β-sitosterol and stigmasterol,among other active compounds,down-regulating the EGFR/STAT3 signaling pathway,enhancing autophagy in cardiomyocytes of IHF mice,and improving left ventricular remodeling.
