APOA5 gene polymorphisms,serum LPS,Spd-L1 and PCT in severe acute pancreatitis patients with secondary infectious pancreatic necrosis
10.11816/cn.ni.2025-240734
- VernacularTitle:重症急性胰腺炎继发感染性胰腺坏死APOA5基因多态性及血清LPS、sPD-L1和PCT水平
- Author:
Fei LIU
1
;
Liqian ZHANG
;
Naikun GAO
;
Zhifei QIAO
;
Lei WANG
;
Chunyan LIU
;
Fulong LI
;
Jie YAO
Author Information
1. 河北北方学院附属第一医院重症医学科,河北 张家口 075000
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis;
Infectious pancreatic necrosis;
Soluble programmed death ligand 1;
Lipase;
Procalcitonin;
Apolipoprotein A5;
Gene polymorphism;
Receiver operating characteristic curve
- From:
Chinese Journal of Nosocomiology
2025;35(5):662-666
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the APOA5 gene polymorphisms,serum lipase(LPS),soluble programmed death ligand 1(sPD-L1)and procalcitonin(PCT)in the severe acute pancreatitis(SAP)patients with secondary infectious pancreatic necrosis(IPN).METHODS A total of 122 patients with SAP who were treated in the First Affiliated Hospital of Hebei North University from Jan.2020 to Nov.2023 were recruited as the research subjects and were divided into the secondary group with 31 cases and the non-secondary group with 91 cases according to the status of secondary IPN.The APOA5 gene polymorphisms were detected for the two groups of patients by means of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The levels of LPS,sPD-L1 and PCT as well as Ranson score were compared between he two groups.The association of the serum LPS,sPD-L1 and PCT with Ranson score was observed by Spearman analysis.The value of the joint detection of LPS,sPD-L1 and PCT in diagnosis of secondary IPN in the SAP patients was analyzed by means of receiver oper-ating characteristic(ROC)curves.RESULTS The frequencies of AA genotype and A allele at rs619054 locus of APOA5 gene were higher in the secondary group than in the non-secondary group(P＜0.05),and the frequency of G allele of the secondary group was lower than that of the non-secondary group(P＜0.05).There were significant differences in the levels of serum LPS,sPD-L1 and PCT as well as Ranson score between the secondary group and the non-secondary group(P＜0.05).Spearman analysis showed that the Ranson score was positively correlated with the levels of serum LPS,sPD-L1 and PCT(r=0.738,0.701,0.721,P＜0.05).ROC curve analysis indicated that the area under the curve(AUC)of the joint detection of the three indexes was higher than that of the single detection in diagnosis of secondary IPN in the SAP patients(P＜0.05),with the sensitivity 90.30％,the specifici-ty 84.60％.CONCLUSIONS The SAP patients with secondary IPN show the high expressions of LPS,sPD-L1 and PCT.The joint detection of the indexes may facilitate the diagnosis of the secondary IPN in the SAP patients.There is association between the levels of LPS,sPD-L1 and PCT and the Ranson score.The rs619054 locus of APOA5 gene may be involved in pathogenesis of secondary IPN in the SAP patients.
