Effects of Gynura divaricate polysaccharide on gouty nephropathy induced by dry yeast combined with adenine in rats
10.3969/j.issn.1001-1528.2025.04.011
- VernacularTitle:白背三七多糖对干酵母联合腺嘌呤所致痛风性肾病大鼠的影响
- Author:
Chun-ting ZHI
1
;
Yu-hua WEI
;
Miao ZHANG
;
Zu-ding LIU
;
Hua ZHU
;
Li-ba XU
Author Information
1. 广西中医药大学,广西南宁 530200
- Publication Type:Journal Article
- Keywords:
Gynura divaricate polysaccharide;
gouty nephropathy;
allopurino;
adenine;
dry yeast
- From:
Chinese Traditional Patent Medicine
2025;47(4):1137-1143
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the protective effect of Gynura divaricate polysaccharide on gouty nephropathy(GN)induced by dry yeast combined with adenine in rats.METHODS Sixty male SD rats were randomly divided into the normal group,the model group,the allopurinol group(42 mg/kg),and the low-dose,medium-dose and high-dose G.divaricate polysaccharide groups(140,280,560 mg/kg).All the rats except those of the normal group were induced into GN models by intragastrical dosing of yeast(5 g/kg)and adenine(100 mg/kg)and intervened with corresponding drug administration simultaneously.After 35 days,the rats had their levels of creatinine(Cr)and uric acid(UA)in serum and urine detected and their fraction excretion of uric acid(FEUA)value determined;their kidney mass and volume measured and their levels of kidney index and density calculated;their renal pathological changes checked by HE staining;their renal GLUT9,URAT1,ABCG2 and OAT1 mRNA expressions dectected by RT-qPCR;and their renal GLUT9,URAT1,ABCG2 and OAT1 protein expressions dectected by Western blot.RESULTS Compared with the model group,each dose of G.divaricate polysaccharide group displayed decreased levels of kidney mass,kidney volume and kidney index(P＜0.01);increased levels of kidney density(P＜0.05,P＜0.01);decreased serum levels of UA and Cr(P＜0.01);increased urine levels of UA and Cr(P＜0.01);increased FEUA value(P＜0.01);decreased GLUT9,URAT1 mRNA and protein expressions(P＜0.05,P＜0.01);and increased ABCG2,OAT1 mRNA and protein expressions(P＜0.05,P＜0.01);and more alleviated renal histological aberrations.CONCLUSION G.divaricate polysaccharide exerts good protective effects against yeast/adenine-induced GN in rats probably through down-regulating protein expression of GLUT9,URAT1 and up-regulating ABCG2 and OAT1.
