Model establishment for quantitative analysis of saponins of Paris polyphylla by near-infrared spectroscopy
10.3969/j.issn.1001-1528.2025.04.001
- VernacularTitle:滇重楼皂苷近红外光谱快速定量分析模型建立
- Author:
Ping XU
1
;
Qi MI
;
Wen-xiu LUO
;
You LU
;
Meng-wen YU
;
Xuan ZHANG
;
Guo-wei ZHENG
;
Chang-gui QIU
;
Jia CHEN
Author Information
1. 云南中医药大学中药学院,云南 昆明 650500
- Publication Type:Journal Article
- Keywords:
Paris polyphylla var.yunnanensis;
saponins;
HPLC;
near infrared spectroscopy;
quantitative analysis model
- From:
Chinese Traditional Patent Medicine
2025;47(4):1069-1076
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a rapid quantitative analysis model for saponins in Paris polyphylla var.yunnanensis(PPY)by near infrared spectroscopy.METHODS The contents of polyphyllins Ⅰ,Ⅱ,Ⅶ and there total content in PPY were determined by HPLC,while spectral data within the range of 10 000 to 4 000 cm-1 were collected.A quantitative analysis model was established by combining these data with partial least squares regression(PLSR).Multivariate scatter correction(MSC)and vector normalization(SNV)were applied prior to further preprocessing the spectra with original,first-order derivative(1stD),or second-order derivative(2ndD)treatments.Lastly,the model was optimized through non-smoothing(NS),Norris Derivative filtering(Nd),and Savitzky-Golay filtering(S-G)method.Model stability was evaluated based on correlation coefficients and variance.The predicted contents of each saponin component in the validation set samples were calculated.RESULTS The contents of polyphyllins Ⅰ,Ⅱ,Ⅶ were 0.42-17.98,0.46-10.44,0.23-3.86 mg/g,respectively.The total content ranged from 2.91 to 22.1 mg/g.The optimal parameters of three saponins were achieved when selecting the MSC+2ndD+S-G pretreatment method.The corresponding ratio of line segment length to segment gap was 13∶5,15∶5,11∶5,with correlation coefficients of 0.982,0.930,0.958,respectively.The root mean square errors of calibration(RMSEC)were 0.702,0.797,0.238,and the root mean square errors of prediction(RMSEP)were 1.120,0.835,0.304,respectively.The optimal parameters for the total content were obtained when selecting the MSC+2ndD+NS pretreatment method,with a correlation coefficient of 0.970,a RMSEC of 1.090,and a RMSEP of 1.740.CONCLUSION This accurate and rapid method can be used for detection of saponin contents in P.Polyphylla.
