Pathogenic bacteria isolated from recurrent spontaneous abortion patients complicated with reproductive tract infection and changes of peripheral blood miR-155 and Th17/Treg cytokines
10.11816/cn.ni.2025-240790
- VernacularTitle:复发性流产合并生殖道感染病原菌及外周血miR-155、Th17/Treg细胞因子变化
- Author:
Shan WANG
1
;
Youyun LI
;
Beibei CHEN
;
Huiling ZHAO
;
Shuai XU
Author Information
1. 新乡市中心医院生殖医学科,河南 新乡 453000
- Publication Type:Journal Article
- Keywords:
Recurrent spontaneous abortion;
Genital tract infection;
Pathogen;
Microribonucleic acid-155;
Helper T cell 17/regulator T cell
- From:
Chinese Journal of Nosocomiology
2025;35(5):692-696
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the distribution of pathogens isolated from the recurrent spontaneous abortion patients complicated with genital tract infection,observe the changes of peripheral blood microribonucleic acid-155(miR-155),helper T cell 17(Th17)/regulatory T cell(Treg)cytokines and analyze the clinical significance.METHODS A total of 102 recurrent spontaneous abortion patients who were complicated with genital tract infec-tion and were treated in Xinxiang Central Hospital from Jan.2022 to Oct.2023 were assigned as the infection group,meanwhile,80 recurrent spontaneous abortion patients who were not complicated with genital tract infec-tion were chosen as the no infection group.The distribution of the pathogens isolated from the patients of the in-fection group was observed.The expression levels of peripheral blood miR-155,Th17/Treg and their cytokines[interleukin(IL)-10,IL-17]were compared between the two groups.The values of the peripheral blood miR-155,Th17/Treg,IL-10 and IL-17 in prediction of genital tract infection in the recurrent spontaneous abortion patients were analyzed by means of receiver operating characteristic(ROC)curves.RESULTS Totally 121 strains of patho-gens were isolated from the 102 recurrent spontaneous abortion patients complicated with genital tract infection,68(56.20％)of which were gram-negative bacteria,45(37.19％)were gram-positive bacteria,and 8(6.61％)were fungi.Escherichia coli was the predominant species of gram-negative bacteria;Staphylococcus aureus was dominant among the gram-positive bacteria;Candida albicans was the predominant species of fungi.There were significant differences in the levels of peripheral blood miR-155,Th17/Treg and cytokines between the infection group and the no infection group(P＜0.05).ROC curve analysis showed that the areas under the curves(AUCs)of peripheral blood miR-155,Th17/Treg,IL-10,IL-17 and the joint detection of the above indexes were 0.891,0.909,0.877,0.869 and 0.994,respectively;the joint detection was superior to the single detection(P＜0.05).CONCLUSIONS The gram-negative bacteria are dominant among the pathogens isolated from the recurrent sponta-neous abortion patients complicated with genital tract infection.The miR-155,Th17/Treg and their cytokines have certain values in prediction of the genital tract infection in the patients with recurrent spontaneous abortion.
