Study on Chemical Constituents and Fingerprints of Hedysari Radix Praeparata Cum Melle and Vinegar Processed Curcumae Rhizoma before and after Compatibility
10.19879/j.cnki.1005-5304.202411454
- VernacularTitle:炙红芪-醋莪术配伍前后化学成分及指纹图谱研究
- Author:
Yuefeng LI
1
;
Fenyu DOU
;
Zhuanhong ZHANG
;
Ruilong LYU
;
Mengna CHAI
;
Dingcai MA
;
Maomao WANG
;
Zhe WANG
;
Xingke YAN
Author Information
1. 甘肃中医药大学药学院,甘肃 兰州 730000;甘肃中医药大学科研实验中心,甘肃 兰州 730000
- Publication Type:Journal Article
- Keywords:
Hedysari Radix Paeparata Cum Melle;
vinegar processed Curcumae Rhizoma;
HPLC;
compatibility;
chemical composition;
fingerprint
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(5):141-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective Through studying the chemical composition changes before and after the compatibility of Hedysari Radix Paeparata Cum Melle(HRPCM)-vinegar processed Curcumae Rhizoma(VPCR);To discuss the significance of the compatibility of HRPCM and VPCR;To establish the fingerprints before and after their compatibility.Methods ZORBAX Eclipse Plus C18 column was used;acetonitrile-0.01%phosphoric acid water was set as mobile phase,with gradient elution;column temperature was 30℃;detection wavelength was 214 nm;sample injection was 10 μL,which was used to investigate the content difference of major chemical components such as vanillic acid,calycosin-O-β-D-glucopyranoside,ononin,calycosin,onocerin,curdione,cincumol and germacrone,and establish the fingerprint of HRPCM,VPCR and HRPCM-VPCR.Results HPLC chromatographic conditions were established for the determination of 8 components in HRPCM-VPCR.Meanwhile,fingerprints were established before and after the compatibility of HRPCM-VPCR.26 common peaks were identified,among which 11 components such as vanillic acid were derived from HRPCM,14 components such as curcuma zedoariae were derived from VPCR,and 1 component was shared by both.Conclusion The material basis of the compatibility of HRPCM-VPCR differs from that of HRPCM and VPCR.The content of most chemical components decreases while the content of some components increases.The established HPLC method for content determination and fingerprint is simple,stable and reproducible,which can be used to evaluate and control the quality of HRPCM and VPCR.
