Effects of different oxygen partial pressure exposure on the content of molecules related to adenosine metabolism pathway and relevant enzyme expressions in the lungs and brains of mice
10.3760/cma.j.cn311847-20211214-00341
- VernacularTitle:不同氧分压暴露对小鼠脑和肺组织腺苷代谢通路分子及相关酶表达的影响
- Author:
Ying LIU
1
;
Zhenbiao GUAN
;
Wenwu LIU
Author Information
1. 烟台市烟台山医院病理科，烟台 264001
- Publication Type:Journal Article
- Keywords:
Oxygen toxicity;
Lung;
Brain;
Adenosine;
Adenosine deaminase;
Adenosine kinase
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2022;29(3):321-325,335
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of exposure to oxygen at different partial pressures on the contents of molecules related to adenosine metabolism and the expressions of relevant enzymes in the lungs and brains of mice.Methods:A total of 36 male mice were randomly divided into normal control group，hyperoxia-induced lung injury（HILI）group，and acute oxygen toxicity（ACOT）group，with 12 mice in each group. In the HILI group，the mice were exposed to oxygen at 0.25 MPa（2.5 ATA）for 6 h；in the ACOT group，the mice were exposed to oxygen at 0.40 MPa（4.0 ATA）for 30 min. Immediately after hyperoxia exposure，all the mice were anesthetized intraperitoneally，and the lungs and brains were harvested for further assays. High performance liquid chromatography（HPLC）was employed for the detection of ATP，ADP，AMP，and adenosine contents；western blotting was performed for the detection of CD37，ADA，and ADK protein expressions.Results:Exposure to oxygen at different partial pressures could increase the contents of adenosine in the brains and lungs to different extents，this may be ascribed to the increased expression of enzyme for the adenosine synthesis and the inhibited expression of enzyme for the adenosine catabolism. Moreover，in the ACOT group，the changes in the molecules in the lungs were more significant than those in the brains.Conclusion:Exposure to oxygen at different partial pressures may affect the adenosine metabolism pathway by increasing the adenosine in the lungs and brains，which may serve as a protective response to hyperoxia exposure，thus protecting the cells against hyperoxia-induced injuries.
