Temozolomide combined with hyperbaric oxygen can inhibit lung cancer A549 cell cycle and apoptosis by regulating the AKT/mTOR signaling pathway
10.3760/cma.j.cn311847-20210428-00135
- VernacularTitle:替莫唑胺联合高压氧通过调控AKT/mTOR信号通路影响人肺癌A549细胞周期及凋亡
- Author:
Jianhui ZHANG
1
;
Caixia ZHANG
;
Siqing YAO
;
Yi LI
;
Lage WEI
;
Yanjun LIU
Author Information
1. 运城市中心医院胸外科，运城 044000
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Lung cancer;
Cell cycle;
AKT/mTOR signaling pathway
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2022;29(2):221-224
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of hyperbaric oxygen（HBO）combined with Temozolomide（TMZ）on the cell cycle and apoptosis of lung cancer A549 cells，and to study its mechanisms.Methods:After the cell culture，the human lung cancer A549 cells were divided into control group，HBO group，TMZ group，and HBO+TMZ group according to the experimental design，and were given，respectively，no treatment，50 μmol/L TMZ for 24 h，0.2 MPa HBO for 3 h，and 0.2 MPa HBO pretreatment for 3 h followed by 50 μmol/L TMZ for 24 h. The cell proliferation was detected by Cell Counting Kit-8（CCK-8）experiment；the cell apoptosis and cell cycle were detected by flow cytometry；the expression of AKT/mTOR signaling pathway proteins in A549 cells was detected by Western blotting.Results:The survival rate of A549 cells in the HBO+TMZ group was significantly lower than those of the other three groups（ P < 0.05 or P < 0.01）. The apoptosis rates in the control group，HBO group，TMZ group，and HBO+TMZ group were（6.73 ± 1.47）%，（8.52 ± 0.87）%，（32.78 ± 3.49）%，and（49.61 ± 5.74）%，respectively；the apoptosis rate in the HBO+TMZ group was significantly higher than those in the other three groups，and the differences were statistically significant（ P < 0.05）. The results of cell cycle experiment showed that the number of A549 cells in G2/M phase was increased in the HBO+TMZ group with a cell percentage of 35.81%，which was significantly higher than those of the other three groups（ P < 0.05）. The expression levels of p-AKT and mTOR proteins in A549 cells in the HBO+TMZ group were significantly lower than those in the other three groups（ P < 0.01）. Conclusion:The combination of HBO and TMZ can greatly improve the inhibition on the proliferation and induce apoptosis of lung cancer A549 cells，which is achieved by regulating the AKT/mTOR signaling pathway to block the A549 cells in the G2/M phase.
