Analysis of the mechanism of acute hypotension:Bioinformatics-driven target screening and pathway mining
10.3969/j.issn.1673-9701.2025.30.005
- VernacularTitle:急性低血压分子机制解析:生物信息学驱动的靶点筛选与通路挖掘
- Author:
Yuting MEI
1
;
Zhou ZHOU
;
Xuan ZHANG
;
Huimin FAN
;
Mengxue ZHOU
;
Xianliang WANG
Author Information
1. 天津中医药大学第一附属医院心血管科国家中医针灸临床医学研究中心,天津 300381;天津中医药大学研究生院,天津 301617
- Publication Type:Journal Article
- Keywords:
Acute hypotension;
GEO database;
Bioinformatics;
Pathological mechanism;
Ribosome dysfunction;
Changes in insulin signal efficacy
- From:
China Modern Doctor
2025;63(30):20-25,101
- CountryChina
- Language:Chinese
-
Abstract:
Objective Based on multi-dimensional bioinformatics technology,the molecular pathological mechanism of acute hypotension is systematically analyzed.Methods Integrate the gene expression profile of the Gene Expression Omnibus database(GSE2401),use limma package(R software)to screen differential genes,and optimize targets through data dimensionality reduction(log2(FC)＞1,P＜0.05);Further combine Gene Ontology,Kyoto Encyclopedia of Genes and Genomes and gene set enrichment analysis pathway enrichment and STRING-Cytoscape(MCODE,CytoHubba plug-in)to build a protein-protein interaction(PPI)network to mine core genes;realize the full-chain analysis of"differential gene-functional pathway-PPI network".Results 676 differential genes(304 upregulated,372 downregulated),ribosome structural components(Rps8,Rps27,Rpl35,etc.)and multiple pathways such as forkhead box protein and cyclic adenosine monophosphate were found to coordinate the regulation of acute hypotension.Conclusion This study uses a low-cost and efficient bioinformatics analysis framework to reveal the association between ribosomal dysfunction,changes in insulin signal efficacy and blood pressure regulation,providing new ideas for targeted therapy.
