The application of nanopore sequencing technology in rapid detection infected pathogens
10.3760/cma.j.cn114452-20250106-00013
- VernacularTitle:纳米孔测序技术在感染病原体快速检测中的应用
- Author:
Xinxin LIU
1
;
Chao WANG
1
;
Xiuru GUAN
1
Author Information
1. 哈尔滨医科大学附属第一医院检验诊断科，哈尔滨150001
- Publication Type:Journal Article
- Keywords:
Nanopore sequencing;
Infections;
Early diagnosis
- From:
Chinese Journal of Laboratory Medicine
2025;48(11):1390-1395
- CountryChina
- Language:Chinese
-
Abstract:
Pathogens, including bacteria, viruses, and fungi, can cause infections and inflammatory responses when invading the human body, posing life-threatening risks in severe cases. Pathogen culturing is considered the gold standard for diagnosing infections, but its time-consuming nature hampers early clinical diagnosis. Polymerase chain reaction offers high sensitivity and specificity but can result in false-negative outcomes. Nanopore sequencing, which features real-time sequencing while unwinding the DNA strands, allows for direct sequencing and immediate analysis. In viral detection, it rapidly obtains pathogen genomes and identifies genetic variations. Direct sequencing of the 16S ribosomal RNA in bacteria and the internal transcribed spacer sequences in fungi enables early diagnosis. It can also detect bacterial resistance, guiding clinical doctors in selecting appropriate antibiotics. Additionally, rapid pathogen detection can be achieved in vector-borne diseases and rare diseases. With advancements in technologies such as deep learning, nanopore sequencing technology is expected to become a powerful tool for early diagnosis of infectious pathogens.
