Exploration on the Protective Effects and Mechanism of Xinkang Granules-Containing Serum in H9C2 Cardiomyocyte Injury Based on cGAS-STING Axis
10.19879/j.cnki.1005-5304.202412546
- VernacularTitle:基于cGAS-STING轴探讨心康冲剂含药血清对H9C2心肌细胞损伤的保护作用及机制
- Author:
Siqin TANG
1
;
Liang LI
;
Bing GUO
;
Qihui XIE
;
Qingqi YIN
;
Qinliang WU
;
Xi YIN
;
Yilin MAO
Author Information
1. 湖南中医药大学第二附属医院,湖南 长沙 410005
- Publication Type:Journal Article
- Keywords:
Xinkang Granules-containing serum;
H9C2 cell;
myocardial injury;
cGAS/STING axis
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(11):99-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect and mechanism of Xinkang Granules-containing serum in adriamycin-induced injury of cardiomyocytes H9C2 based on cGAS-STING signaling axis.Methods Adriamycin was used to induce the H9C2 cells injury model.The cells were divided into normal group,model group,Xinkang Granules group and inhibitor group.After 24 hours of intervention,the CCK-8 method was used to detect cell survival rate,the DCFH-DA fluorescent probe was used to detect the content of cell reactive oxygen species(ROS),cell apoptosis rate was detected by flow cytometry,ELISA was used to detect the content of tumor necrosis factor-α(TNF-α)in cell supernatant,colorimetry was used to detect lactate dehydrogenase(LDH)in cells,RT-qPCR was used to detect the expression of mitochondrial transcription factor A(TFAM),cyclic guanosine-adenylate synthetase(cGAS),stimulator of interferon genes(STING)and TNF-α mRNA,Western blot and immunofluorescence were used to detect the protein expressions of cGAS and STING.Results Compared with the normal group,cell survival rate in the model group was significantly reduced(P<0.01),the ROS content was significantly increased(P<0.01),the apoptosis rate significantly increased(P<0.01),the content of TNF-α in the supernatant significantly increased(P<0.01),the activity of LDH significantly increased(P<0.01),the expression of TFAM mRNA significantly decreased(P<0.01),and the expressions of TNF-α,cGAS,STING mRNA and the protein expression of cGAS and STING significantly increased(P<0.01).Compared with the model group,cell survival rate in Xinkang Granules group and inhibitor group significantly increased(P<0.01),the ROS content significantly decreased(P<0.01),the apoptosis rate significantly decreased(P<0.01),the content of TNF-α in supernatant significantly decreased(P<0.01),the activity of LDH significantly decreased(P<0.01),the expression of TFAM mRNA significantly increased(P<0.01),and the expressions of TNF-α,cGAS,STING mRNA and the protein expressions of cGAS and STING significantly decreased(P<0.05,P<0.01).Conclusion Xinkang Granules have a protective effect on adriamycin-induced H9C2 cardiomyocytes,which may be related to the inhibition of cGAS/STING axis activation and the secretion of inflammatory factors.
