The application progress of CRISPR/Cas system biosensors in pathogen detection
10.3760/cma.j.cn114452-20250711-00403
- VernacularTitle:CRISPR/Cas系统的生物传感器在病原体检测中的应用进展
- Author:
Zhengjiang WU
1
;
Tao ZHANG
;
Kai ZHANG
;
Kun HAN
Author Information
1. 中国科学院苏州生物医学工程技术研究所，苏州 215163
- Publication Type:Journal Article
- Keywords:
CRISPR/Cas system;
Biosensor;
Pathogen
- From:
Chinese Journal of Laboratory Medicine
2025;48(10):1278-1290
- CountryChina
- Language:Chinese
-
Abstract:
Pathogen detection is the key to the early diagnosis, prevention and control of infectious diseases. The clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-related protein (Cas) system (CRISPR/Cas) has become an important tool in the field of pathogen detection due to its high specificity, programmability and trans-cleavage activity. Biosensors based on CRISPR/Cas systems are continuously evolving. By integrating with various novel detection strategies, they have significantly reduced detection costs and time, greatly enhanced the simplicity of operation and the practicality of the platform, and demonstrated broad clinical application prospects in the field of pathogen diagnosis.
