Overcoming bottlenecks in CRISPR multiplexed detection: from One-Pot approaches to Logic-Gate architectures
10.3760/cma.j.cn114452-20250623-00362
- VernacularTitle:“一锅式”至“逻辑门”的CRISPR多重检测策略及瓶颈突破
- Author:
Mingmin SHI
1
;
Dianwei LIU
;
Xueliang WANG
;
Rui WANG
Author Information
1. 复旦大学附属浦东医院医学科研与创新中心；复旦大学人类表型组研究院，上海200438
- Publication Type:Journal Article
- Keywords:
CRISPR/Cas system;
Multiplex detection;
One-pot strategy;
Spatial separation;
Logic gate
- From:
Chinese Journal of Laboratory Medicine
2025;48(10):1271-1277
- CountryChina
- Language:Chinese
-
Abstract:
The CRISPR/Cas system has emerged as a promising platform for multiplex nucleic acid detection in recent years. Compared with traditional methods such as qPCR and NGS, CRISPR-based assays offer distinct advantages, including simplicity, high sensitivity, and low cost, making them particularly suitable for pathogen screening and early cancer diagnosis. To address the core challenges of CRISPR-based multiplex detection, three types of strategies have been developed: one-pot, spatial separation, and logic gate.These strategies enable simultaneous recognition of multiple targets and effective signal amplification. Applications span viral detection, antimicrobial resistance profiling, and tumor biomarker analysis. Despite persistent challenges such as signal cross interference and system complexity, the technology is evolving rapidly and holds strong potential for clinical translation.
