CRISPR/Cas-based molecular diagnostics: application bottlenecks and breakthrough strategies analysis
10.3760/cma.j.cn114452-20250814-00464
- VernacularTitle:CRISPR/Cas分子诊断技术：应用瓶颈与突破路径分析
- Author:
Fenyong SUN
1
;
Jingqi CHEN
;
Xiaoli ZHU
Author Information
1. 同济大学附属上海市第十人民医院检验科，上海200072
- Publication Type:Journal Article
- Keywords:
CRISPR/Cas;
Molecular diagnostics;
Pathogen detection;
Point-of-care testing
- From:
Chinese Journal of Laboratory Medicine
2025;48(10):1261-1263
- CountryChina
- Language:Chinese
-
Abstract:
Leveraging the 'trans-cleavage′ activity of Cas proteins, the CRISPR/Cas system has successfully transitioned from a gene-editing tool to a new-generation molecular diagnostic platform. This technology demonstrates notable advantages in point-of-care testing of biomarkers, including rapid detection, operational simplicity, and portability. However, it still faces critical challenges such as insufficient sensitivity and limited multiplex detection capability. Recent studies indicate that through innovative strategies including protein engineering, microfluidic integration, and artificial intelligence algorithm optimization, CRISPR/Cas-based diagnostic technology is advancing molecular diagnostics towards field-deployable and intelligent systems, thereby providing novel solutions for intelligent on-site biomarker screening.
