Applications and research progress of single-molecule immunoassay in disease diagnosis and treatment
10.3760/cma.j.cn114452-20241012-00553
- VernacularTitle:单分子免疫检测技术在疾病诊疗中的应用与研究进展
- Author:
Junyi WU
1
;
Hui YUAN
1
Author Information
1. 首都医科大学附属北京安贞医院检验科，北京 100029
- Publication Type:Journal Article
- Keywords:
Early diagnosis;
Biotechnology;
Biological markers
- From:
Chinese Journal of Laboratory Medicine
2025;48(9):1248-1253
- CountryChina
- Language:Chinese
-
Abstract:
Single-molecule immunoassay is a "digital" immunodetection method that confines immune complexes within an extremely small volume, achieving absolute signal quantification. With a lower detection limit reaching femtograms per milliliter (fg/ml), it offers high sensitivity. This technology is suitable for precision medical testing, facilitating rapid and accurate quantification of low-abundance substances in patient blood samples. At present, single-molecule immunoassay has demonstrated significant application values in the fields of neurodegenerative diseases, cancers, infectious diseases, and cardiovascular diseases. However, it still faces challenges in clinical applications, such as high costs and operational complexity. In the future, further researches are needed to braoden the applications of single-molecule immunoassay to other disease areas. Meanwhile, as this technology progresses into clinical laboratories, it is imperative to improve the interpretation of testing results, clinical validation, and the standardization of testing procedures.
