Diagnostic value of peripheral blood cell analysis-derived 5 new parameters on chronic hepatitis B, liver cirrhosis and hepatocellular carcinoma
10.3760/cma.j.cn114452-20240730-00419
- VernacularTitle:基于外周血细胞分析的5项新参数在慢性乙型肝炎、肝硬化和肝细胞癌中的诊断价值
- Author:
Xinyao YANG
1
;
Xin YANG
;
Xinrong LU
;
Qunfang HUANG
;
Bin YANG
;
Can LIU
;
Qishui OU
;
Zhen XUN
Author Information
1. 福建医科大学附属第一医院检验科，福建省检验医学重点实验室，福建医科大学基因诊断研究中心，福建省临床免疫学检验临床医学研究中心，福州 350005
- Publication Type:Journal Article
- Keywords:
Chronic hepatitis B;
Liver cirrhosis;
Hepatocellular carcinoma;
Blood cells;
Diagnosis
- From:
Chinese Journal of Laboratory Medicine
2025;48(7):917-923
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the laboratory diagnostic value of 5 new blood routine indexes in chronic hepatitis B (CHB), liver cirrhosis and hepatocellular carcinoma (HCC).Methods:The retrospective study included 65 patients with chronic HBV infection, 72 patients with liver cirrhosis and 163 patients with HCC recruited at Liver Disease Center in First Affiliated Hospital of Fujian Medical University, as well as 52 healthy controls recruited from the Physical Examination Center of the First Affiliated Hospital of Fujian Medical University from October 2022 to April 2023. Five new parameters [early granulated cell percent (EGC%), early granulated cell absolute count (EGC#), microcytic anemia factor (MAF), leukocyte estimate(corrected)from the DIFF optical channel (WDOP) and leukocyte estimate(corrected)from the NRBC optical channel (WNOP)] were detected by UniCel DxH 900 blood cell analyzer. Univariate analysis of the expression levels of the 5 new parameterswere compared among CHB, liver cirrhosis and HCC groups. Pearson correlation analysis was used to explore the correlation between the 5 new parameters and HBV-related markers in CHB and Child-Pugh score in liver cirrhosis. Receiver operating characteristic (ROC) curve and AUC were used to estimate the diagnostic capacity of the 5 new blood routine indexes in CHB, liver cirrhosis and HCC.Results:In patients with CHB, the levels of EGC% ( Z=4.613, P<0.001) and EGC# ( Z=4.220, P<0.001) were higher than those of healthy controls; EGC# was positively correlated with HBsAg and HBeAg (both P<0.05). In patients with cirrhosis, the level of MAF ( Z=-4.928, P<0.001) was lower than that of healthy controls, and Child-Pugh score was found to be negatively correlated with MAF ( r=-0.349, P<0.05). In HCC patients, WDOP ( Z=2.45, P=0.017) and WNOP ( Z=2.90, P=0.017) levels were higher in patients with tumor volume>3 cm 3 than those in patients with volume ≤3 cm 3. The AUCs of combination of 5 new parameters to diagnose CHB, liver cirrhosis and HCC were 0.901 (95% CI 0.830-0.973, P<0.001), 0.946 (95% CI 0.909-0.984, P<0.001), and 0.904 (95% CI 0.858-0.950, P<0.001). Conclusions:The 5 new parameters based on peripheral blood cell analysis have good clinical value in the diagnosis of CHB, liver cirrhosis and HCC diseases.
