Application of tumor specific protein 70 targeted enrichment of circulating tumor cell sequencing technology in non-small cell lung cancer
10.3760/cma.j.cn114452-20250422-00247
- VernacularTitle:靶向富集SP70的循环肿瘤细胞测序技术在非小细胞肺癌中的应用
- Author:
Lixia ZHANG
1
;
Yutong ZHOU
1
;
Shuxian YANG
1
;
Qiong ZHU
1
;
Jian XU
1
;
Yuan MU
1
;
Chunrong GU
1
;
Huanyu JU
1
;
Wei ZHANG
1
;
Shiyang PAN
1
Author Information
1. 南京医科大学第一附属医院检验学部，国家医学检验临床医学研究中心分中心，南京 210029
- Publication Type:Journal Article
- Keywords:
Liquid biopsy;
Circulating tumor cell;
Next generation sequencing;
Tumor specific protein 70
- From:
Chinese Journal of Laboratory Medicine
2025;48(6):737-742
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish tumor specific protein (SP70) targeted tumor cell enrichment technology and to assess applicational value of next-generation sequencing (NGS) analysis for enriched circulating tumor cell (CTC) in precision medicines of non-small cell lung cancer (NSCLC).Methods:The monoclonal antibody NJ001 was covalently coupled to the surface of magnetic beads to build targeted magnetic bead enrichment technology based on SP70. The limit of detection, coincidence rate, interference experiment, recovery test and clinical performance were evaluated. From March 2016 to August 2017, NGS analysis with or without pre-treatment of targeted enrichment for serous fluids of 43 NSCLC in the First Affiliated Hospital with Nanjing Medical University were compared (Kappa or Fisher exact test).Results:The CTC enrichment technology based on SP70 targeted immunomagnetic beads can specifically enrich tumor cells. The limit of detection was 10 4 SPC-A1 cells/L, and the coincidence rate, sensitivity and specificity were 100% (3/3). The endogenous interfering substances such as red blood cells, hemoglobin, white blood cells, epithelial cells and triglycerides had no interfering effects, as well as the exogenous interfering substances such as EDTA-K2, cefoxitin, carboplatin and paclitaxel. The recovery rate was 56.0% (56 000/100 000). A total of 30 gene mutations including 65 loci were found in 43 NSCLC under SP70 targeted enrichment, with a higher detection rate compared with unenrichment method [95.0% (19/20) vs 65.0% (13/20), χ 2=5.625, P=0.044]. Conclusion:In this study, SP70-targeted enriched CTC liquid biopsy method was established, with higher sensitivity and specificity of NGS detection than unenrichment method.
