Progress of clinical detection methodology of varicella vaccine serum antibody
10.3760/cma.j.cn114452-20250303-00125
- VernacularTitle:水痘疫苗血清抗体临床检测方法学进展
- Author:
Yaru QUAN
1
;
Yanli DAI
;
Changgui LI
;
Kangwei XU
;
Jizong JIA
Author Information
1. 中国食品药品检定研究院，北京 102629
- Publication Type:Journal Article
- Keywords:
Varicellovirus;
Evaluation studies;
Fluorescent antibody to membrane antigen method;
Glycoprotein enzyme-linked immunosorbent assay method
- From:
Chinese Journal of Laboratory Medicine
2025;48(6):785-790
- CountryChina
- Language:Chinese
-
Abstract:
Varicella is a common infectious disease caused by varicella-zoster virus (VZV), with high transmissibility and pathogenicity, and vaccination is a better preventive measure. The clinical diagnosis of varicella, the clinical immunogenicity evaluation of varicella vaccine, the continuous monitoring of the immune effect after marketing and the epidemiological investigation all require the detection of the antibody level of VZV in patients. Fluorescent antibody to membrane antigen method (FAMA) is the gold standard for detection of VZV antibody in serum, possessing advantages of high sensitivity and good specificity, playing an important role in detection of VZV antibody. However, the defects of FAMA operation complexity and low throughput are also troubling the clinical testing personnels. This article reviews research progress both at home and abroad on the technology of VZV serum antibody detection. The advantages and limitations of the current detection methods are discussed and the future development direction are highlighted on the dectection of VZV serum antibody IgG.
