Chemical constituents from ethyl acetate fraction of Balanophora harlandii and their tyrosinase inhibitory activity
10.3969/j.issn.1001-1528.2025.10.018
- VernacularTitle:鹿仙草乙酸乙酯部位化学成分及其酪氨酸酶抑制活性研究
- Author:
Zhang-xian CHEN
1
;
Hai-ming WANG
1
;
Yun-tao ZHANG
1
;
Mao-xin DENG
1
;
Kui-lin ZHU
1
;
Jin-lian ZOU
1
;
Jian WANG
1
;
Shan-shan WEI
1
;
Hong-ping HE
1
;
Fa-wu DONG
1
Author Information
1. 云南中医药大学中药学院,云南省南药可持续利用重点实验室,云南 昆明 650500
- Publication Type:Journal Article
- Keywords:
Balanophora harlandii Hook.f.;
ethyl acetate fraction;
chemical constituents;
isolation and identification;
tyrosinase inhibitory activity
- From:
Chinese Traditional Patent Medicine
2025;47(10):3290-3297
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from ethyl acetate fraction of Balanophora harlandii Hook.f.and their tyrosinase inhibitory activity.METHODS Separation and purification were performed using silica gel,MCI,ODS,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The monophenolase inhibitory activity was determined by the tyrosinase-catalyzed oxidation of L-tyrosine.RESULTS Twenty-four compounds were isolated and identified as sesamin(1),methyl caffeate(2),quercetin(3),5,7-dihydroxychromanone(4),methyl 3,4-dihydroxybenzoate(5),esculetin(6),kaempferol(7),naringenin(8),pyrogallic acid(9),pinosylvin(10),methyl propionate(11),caffeic acid(12),saccharinol(13),ferulic acid(14),trans-p-hydroxycinnamic acid(15),cinnamic acid(16),vanillic acid(17),vanillin(18),4-hydroxyacetophenone(19),4-hydroxybenzaldehyde(20),apigenin(21),(-)-isolariciresinol(22),(-)-secoisolariciresinol(23)and meso-2,3-di(3′,4′-methylenedioxybenzyl)butane-1,4-diol(24).The IC50 values of compounds 3,5,7,8,19,and 20 ranged from(0.246 5±0.028 3)to(1.278 2±0.021 3)mmol/L.CONCLUSION Compounds 1-9、11、15、17-21、24 are isolated from this plant for the first time,and 1,6,9,17-19,24 are first isolated from genus Balanophora.Compounds 3、5、7、8、19 and 20 have tyrosinase inhibitory activity.
