Screening and biological characteristics of bacteriophage HN_Aba_01 against multidrug-resistant Acinetobacter baumannii
10.11816/cn.ni.2025-251268
- VernacularTitle:多重耐药鲍曼不动杆菌噬菌体HN_Aba_01的筛选及生物学特性
- Author:
Hanwang ZHOU
1
;
Lin CONG
1
;
Mei YU
1
;
Huihui KUANG
1
;
Yue ZHANG
1
;
Hongyan HU
1
Author Information
1. 解放军总医院海南医院检验科,海南三亚 572000
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Bacteriophage;
Biological characteristic;
Whole-genome sequencing;
Muti-drug resistance;
Cantaminated water
- From:
Chinese Journal of Nosocomiology
2025;35(20):3089-3094
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the biological characteristics and genomic features of highly lytic bacteriopha-ges isolated from sewage in tropical hospitals and provide references for the hospital-associated infection prevention and control of multidrug-resistant Acinetobacter baumannii(MDR-Ab).METHODS With MDR-Ab as the host bacterium,bacteriophages were isolated from sewage.Transmission electron microscopy was employed to observe their morphology,and determine the optimal multiplicity of infection(MOI),one-step growth curve and environ-mental stability.Whole-genome sequencing and bioinformatics analysis were conducted to annotate functional genes and construct a phylogenetic tree.RESULTS A virulent bacteriophage,HN_Aba_01,was isolated.Elec-tron microscopy revealed that it belonged to the Myoviridae family,with a head diameter of 50 nm and a tail length of 90 nm.This bacteriophage exhibited strong lytic activity,with an optimal MOI of 0.000 000 1,a latent period of 5 minutes and a lysis yield of 15 PFU/cell.It remained stable at temperatures ranging from 4 ℃ to 60 ℃and pH values from 3 to 10.Genomic analysis identified 85 ORFs,including lyase,perforin and depolymerase genes.It shared 98.12％identity with the bacteriophage AbP2 genome(reference genome)and was classified into the Obolenskvirus genus.CONCLUSIONS The bacteriophage isolated from tropical hospital sewage,with high lyt-ic activity and good environmental adaptability,can be used for the hospital-associated infection prevention and control of MDR-Ab.
