Advances in research on PCR and mNGS in diagnosing invasive aspergillosis and mucormycosis
10.11816/cn.ni.2025-246588
- VernacularTitle:PCR和mNGS诊断侵袭性曲霉病和毛霉病的研究进展
- Author:
Wei WANG
1
;
Hua ZHOU
;
Lin CHEN
Author Information
1. 绍兴市中心医院医共体总院呼吸与危重症医学科,浙江绍兴 312030
- Publication Type:Journal Article
- Keywords:
Molecular diagnosis technology;
Invasive mucormycosis;
Invasive aspergillosis;
Metagenomic next-generation sequencing;
Polymerase chain reaction;
Mucor;
Aspergillus
- From:
Chinese Journal of Nosocomiology
2025;35(19):3025-3030
- CountryChina
- Language:Chinese
-
Abstract:
Major invasive fungi include Aspergillus,Mucor,Candida,Cryptococcus and Pneumocystis,with As-pergillus and Mucor being the primary pathogens contributing to high mortality in IFD.Traditional diag-nosic methods such as fungal culture and histopathological test are difficult to meet the requirement for early diag-nosis due to their limitations like low sensitivity and long test time.There is an urgent clinical need for molecular diagnostic methods with high sensitivity and specificity to differentiate colonization from invasive infection,appli-cable to various samples(e g.,body fluids,tissues,blood).Additionally,ideal detective technologies should have the characteric of low cost,easy maintenance,and iterative updatability.As emerging molecular diagnostic tech-nologies,polymerase chain reaction(PCR)and metagenomic next-generation sequencing(mNGS)demonstrate significant advantages in diagnosing invasive aspergillosis and mucormycosis due to their high sensitivity and speci-ficity.This paper reviews the current applications,advantages,limitations and future directions of PCR and mNGS in diagnosing invasive aspergillosis and mucormycosis,providing insights into their potential.
