A case of ABO complicated blood group caused by Tnpolyagglutination red blood cells
10.3760/cma.j.cn114452-20240718-00382
- VernacularTitle:Tn多凝集红细胞导致的ABO疑难血型1例
- Author:
Xinyu HUANG
1
;
Xiaozhen HONG
;
Xianguo XU
;
Bing ZHANG
;
Zuonian XIE
;
Faming ZHU
Author Information
1. 浙江中医药大学医学技术与信息工程学院，杭州 310053
- Publication Type:Journal Article
- Keywords:
ABO blood group;
Phytohemagglutinin;
Erythrocyte agglutination;
Gene sequencing
- From:
Chinese Journal of Laboratory Medicine
2025;48(5):662-665
- CountryChina
- Language:Chinese
-
Abstract:
A 90-year-old female patient with novel coronavirus infection, severe pneumonia, and no history of blood transfusion andtransplantation.The mixed appearance phenomenon appeared in the admission blood group identification, and was sent the sample to our laboratory for difficult blood group identification. In the tube saline method, the patient′s red blood cells were positively reacted with 2 monoclonal anti-A and 5 human anti-A reagents.In the microcolumn gel method, the patient′s red blood cells showed 2 positive and 2 negative reactions with monoclonal anti-A and 5 positive and 1 negative reactions with human anti-A. The patient ′s red blood cells showed negative reaction with peanuts in phytohemagglutinin, and positive reaction with double flower lentils, wild soybeans and a string of purples. The patient ′s red blood cells treated with papain showed negative reaction with all monoclonal anti-A reagents, human anti-A and phytohemagglutinin. The patient ′s ABO gene was sequenced as ABO * B.01/O.01.02, but C1GALT1C1 gene mutation was not founded in the gDNA of the whole blood sample.It is speculated that the exposure of Tn antigen on the patient ′s red blood cells leads to red blood cells polyagglutination, resulting in ABO blood group inconsistency.
