Efficiency of targeted next-generation sequencing in diagnosis of pathogens causing acute respiratory tract infections:a meta-analysis
10.11816/cn.ni.2025-241984
- VernacularTitle:靶向高通量测序技术对急性呼吸道感染病原体诊断效能的Meta分析
- Author:
Zixuan XU
1
;
Jinrong XIA
;
Feiyang XU
;
Guanjie WANG
;
Zihan PU
;
Longfeng JIANG
;
Wensen CHEN
;
Bijie HU
;
Yue YANG
Author Information
1. 南京医科大学第一附属医院感染管理处,江苏南京 210029;南京医科大学第一临床医学院,江苏南京 210029
- Publication Type:Journal Article
- Keywords:
Targeted next-generation sequencing;
Respiratory tract infection;
Pathogen;
Meta-analysis;
Diagnostic value
- From:
Chinese Journal of Nosocomiology
2025;35(18):2731-2735
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE T o explore the value of targeted next-generation sequencing(t-NGS)in diagnosis of respir-atory tract pathogens through meta-analysis so as to provide reference for clinical application.METHODS PubMed database,Web of Science database,Wanfang database,CNKI database and Sinomed database were retrieved,and the time period of retrieval ranged from Jan.2010 to May 2024.The literatures were screened out based on the es-tablished standards.The quality was assessed by QU ADAS-2,the risk of bias graph was drawn by Revman 5.4,and the statistical analysis was performed by Stata 16.0.RESULTS A total of 9 literatures were included in the study.The result of meta-analysis showed that the heterogeneity test Q for sensitivity was 268.21,P＜0.01,I2=97.02％,with the heterogeneity test Q for specificity 210.04,P＜0.01,I2=96.19％,the combined sensitivity 0.88(95％CI:0.62 to 0.97),combined specificity 0.68(95％CI:0.41 to 0.86),combined positive likelihood ratio 2.72(95％CI:1.44 to 5.15),combined negative likelihood ratio 0.18(95％CI:0.06 to 0.53),combined diagno-sis score 2.74(95％CI:1.68 to 3.80),and combined diagnostic odds ratio 15.44(95％CI:5.34 to 44.66).The area under synthesize receiver operating characteristic(SROC)curve(AUC)was 0.85(95％CI:0.82 to 0.88).The result of Deeks funnel plot showed that P was 0.99,indicating that there was no obvious publication bias.CONCLUSIONS The sensitivity of tNGS is high in detection of the pathogens causing acute respiratory tract infection,the specificity needs to be improved,but its comprehensive ability is satisfactory.It has certain val-ue in early clinical diagnosis.
