The degree of refinement of immune cell subsets detection by multi-parameter flow cytometry should advance with the times
10.3760/cma.j.cn114452-20250206-00058
- VernacularTitle:多参数流式细胞术检测免疫细胞亚群精细化程度应与时俱进
- Author:
Hui WANG
1
;
Wei CUI
Author Information
1. 河北燕达陆道培医院检验科，廊坊065201
- Publication Type:Journal Article
- Keywords:
Flow cytometry;
Refined;
Immune cell subsets;
Unconventional T cell
- From:
Chinese Journal of Laboratory Medicine
2025;48(5):549-558
- CountryChina
- Language:Chinese
-
Abstract:
Multi-parameter flow cytometry (MFC) for detecting immune cell subsets is an important clinical assay. With the increasingly widespread application of immune cell subset analysis in the diagnosis and treatment of immune-related diseases and health management, new problems and challenges have emerged for refined detection work. The development trend of immune cell subset detection is refined hierarchical analysis, that is, with the increase of markers, more parameters are obtained. On one hand, the in-depth analysis of major immune cell subsets is becoming more and more refined; on the other hand, the phenotypes and functions of unconventional T cells and minor subsets of major immune cells are further recognized. In clinical work, it should be gradually deepened according to the needs of disease diagnosis and treatment. Therefore, it is necessary to understand the changes of immune cell subsets in common diseases such as infectious diseases, autoimmune diseases, and solid tumors, and to select optimized markers, corresponding gradient and depth detection panels in combination with clinical needs and laboratory MFC detection capabilities, and gradually improve them. It should be noted that refined immune cell subset analysis should adopt a consistent analysis panel in one laboratory, increase the number of obtained cells, use appropriate gating methods, subset markers, boundaries and internal controls, and determine reference intervals and clinical diagnostic thresholds.
