The correlation of HBV DNA levels with peripheral blood lymphocyte subsets and IL-6 in patients with chronic hepatitis B
10.3760/cma.j.cn114452-20241030-00597
- VernacularTitle:慢性乙型肝炎患者HBV DNA水平与外周血淋巴细胞亚群及IL-6的相关性分析
- Author:
Peiran LI
1
;
Chengrong BIAN
1
;
Bo LI
1
;
Juling ZHANG
1
;
Ning YANG
1
;
Wei HONG
1
;
Xiuling HE
1
;
Lifang XIA
1
;
Yeli HE
1
;
Bo′an LI
1
Author Information
1. 解放军总医院第五医学中心检验科，北京 100039
- Publication Type:Journal Article
- Keywords:
Hepatitis B, chronic;
HBV DNA viral load;
Lymphocyte subsets;
Interleukin-6
- From:
Chinese Journal of Laboratory Medicine
2025;48(4):484-489
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the correlation of different HBV DNA loads with peripheral blood lymphocyte subsets and interleukin-6 (IL-6) in patients with chronic hepatitis B.Methods:A cross-sectional study was conducted. A total of 519 patients with chronic hepatitis B admitted to the Fifth Medical Center of the General Hospital of the People′s Liberation Army from April 2019 to June 2024 were included. The patients were divided into high, medium, and low viral load groups and a negative group based on HBV DNA load. Another 100 healthy individuals who underwent physical examinations during the same period were recruited as the control group. The quantities of peripheral blood lymphocyte subsets and IL-6 levels were compared among the groups. Meanwhile, alanine aminotransferase (ALT) levels were recorded and compared among the groups. The correlation of HBV DNA levels with lymphocyte subsets and IL-6 was analyzed using Pearson correlation analysis.Results:HBV DNA loads were negatively correlated with the counts of CD3 +, CD4 +, CD8 +, CD19 +, and CD56 + lymphocyte subsets (correlation coefficients r were -0.483, -0.508, -0.524, -0.573, and -0.561, respectively; all P<0.001) and positively correlated with IL-6 levels ( r=0.862, P<0.05). Compared with the control group, the counts of each lymphocyte subset were higher in the high, medium, and low viral load groups ( P<0.05). In the HBV DNA-negative chronic hepatitis B group, the counts of CD8 + and CD19 + lymphocyte subsets were significantly higher [712.32(526.00,898.64) and 495.62(345.74,645.50) cells/μl] than those in the control group [612.10(479.89,744.31) and 470.32 (396.00,544.64) cells/μl] ( P<0.05). Conclusion:The degree of HBV replication activity in patients with chronic hepatitis B is associated with the immune status of the body, and negatively correlated with the quantities of lymphocyte subsets and positively correlated with IL-6 levels.
