Virulence genes and molecular epidemiological characteristics of extensively drug-resistant hypervirulent Klebsiella pneumoniae strains carrying blaKPC-2
10.11816/cn.ni.2025-250139
- VernacularTitle:携带blaKPC-2的泛耐药高毒力肺炎克雷伯菌的毒力和分子流行病学特征
- Author:
Zhijun HU
1
;
Huifeng CHONG
;
Jizhong CHEN
;
Hui ZHOU
;
Juanjuan ZHU
;
Kai PAN
;
Shifang JIANG
Author Information
1. 铜陵市人民医院临床检验中心,安徽铜陵 244000
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
Extensively drug-resistant;
Hypervirulent;
Drug resistance gene;
Whole-ge-nome sequencing;
Molecular epidemiology
- From:
Chinese Journal of Nosocomiology
2025;35(17):2613-2617
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To understand the drug resistance genes,virulence genes and molecular epidemiological characteristics of extensively drug-resistant hypervirulent Klebsiella pneumoniae(XDR-hvKP)strains causing hospital-associated infection.METHODS The clinical isolates of XDR-hvKP were collected from Tongling People's Hospital from Jul.2020 to Dec.2022.The strains were identified by matrix-assisted laser desorption ionization-time-of-flight(MALDI-TOF)mass spectrometry,the common drug resistance genes and virulence genes were an-alyzed by Sanger sequencing,the capsular serotypes were determined by wzi gene sequencing;the drug resistance genes,virulence genes and ST subtypes were observed by means of whole-genome sequencing(WGS)technique.RESULTS Totally 18 strains of XDR-hvKP were collected,55.56％(10/18)of which were isolated from blood specimens,and 61.11％(11/18)were isolated from critical care medicine department.Sanger sequen-cing analysis showed that all of the strains carried blaKPC-2 drug resistance gene;rmpA2(100.00％)and rmpA,i-roN,iutA(94.44％,17/18)were the major virulence genes carried by the strains.WGS analysis indicated that all of the 18 XDR-hvKP isolates carried multiple drug resistance genes such as blaKPC-2 carbapenemase and the viru-lence genes like capsule(rmpA/rmpA2),aerobacterin(iucABCD-iutA),Salmonella(iroN)and yersinin(ybt).All of the ST subtypes were ST 11,and all of the capsular serotypes were KL 64.CONCLUSIONS The ST11-KL64 type XDR-hvKP strains carry blaKPC-2;rmpA,rmpA2,iroN and iutA are the major virulence genes.It is necessary to strengthen the monitoring of key population of the key departments and make joint efforts of multiple departments to contain the transmission of the strains.
