Aldehyde-modified pluronic F127 for loading rapamycin to prepare nanoparticles to inhibit the expansion of abdominal aortic aneurysms
10.3760/cma.j.cn112434-20250307-00072
- VernacularTitle:醛基化改性普朗尼克F127包载雷帕霉素制备纳米粒抑制小鼠腹主动脉瘤
- Author:
Ying WANG
1
;
Wen CHEN
;
Rong QI
Author Information
1. 石河子大学药学院 新疆植物药资源利用教育部重点实验室，新疆石河子 832003
- Publication Type:Journal Article
- Keywords:
Abdominal aortic aneurysm;
Rapamycin;
Aldehyde-modified Pluronic F127;
Nanoparticles
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2025;41(7):427-434
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Preparation of rapamycin nanoparticles inhibits abdominal aortic aneurysm (AAA)in mice.Methods:Aldehyde-modified pluronic F127 encapsulating rapamycin (RAPA) was used to prepare nanoparticles (AMPF@RAPA nanoparticles), which were then characterized and analyzed.Elastase from porcine pancreas was used to induce AAA in C57BL/6J wild-type mice. Rapamycin was administered via intraperitoneal injection, and AMPF@RAPA nanoparticles were administered via intravenous injection. Hematoxylin and eosin staining and elastica van gieson staining were used for histological evaluation of the AAA in mice.Results:Compared with the model group, RAPA intraperitoneal injection significantly reduced the abdominal aorta expansion ratio, with a relative reduction of 40.3% in the abdominal aorta diameter. The particle size of AMPF@RAPA nanoparticles was approximately 104 nm, with an encapsulation efficiency of (71.3±0.7)% and a drug loading of (18.4±0.2)%. Compared with the model group, AMPF@RAPA nanoparticles intravenous injection significantly reduced the abdominal aorta expansion ratio, with a relative reduction of 51% in the abdominal aorta diameter.Conclusion:The intravenous injection of AMPF@RAPA nanoparticles significantly inhibited the development of AAA in mice, demonstrating superior efficacy compared to the intraperitoneal injection of rapamycin.
