Effects of Zuogui Jiangtang Qingzhi Prescription on CD9,CD63 and Apoptosis of Liver Tissue in Type 2 Diabetes Mellitus Mice Combined with Non-alcoholic Fatty Liver Disease
10.19879/j.cnki.1005-5304.202501454
- VernacularTitle:左归降糖清脂方对2型糖尿病合并非酒精性脂肪性肝病小鼠肝组织CD9、CD63及凋亡的影响
- Author:
Jiahui MA
1
;
Chunwei ZHU
1
;
Cong CHEN
1
Author Information
1. 贵州中医药大学,贵州 贵阳 550025
- Publication Type:Journal Article
- Keywords:
Zuogui Jiangtang Qingzhi Prescription;
type 2 diabetes mellitus;
non-alcoholic fatty liver disease;
exosomes;
apoptosis;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(10):100-106
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular mechanism of Zuogui Jiangtang Qingzhi Prescription in improving type 2 diabetes mellitus(T2DM)with non-alcoholic fatty liver disease(NAFLD).Methods Totally 40 8-week-old MKR mice were selected and randomly divided into blank group,model group,Chinese medicine group and positive drug group,with 10 mice in each group.Additionally,10 FVB mice of the same age were set as the normal group.Except for the normal group and the blank group,all other groups were fed with a high-fat diet for 8 weeks.The Chinese medicine group and the positive drug group were given corresponding drugs by gavage for 8 weeks after modeling.Fasting blood glucose(FBG)was measured using a blood glucose meter,and the serum contents of alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglycerides(TG)and total cholesterol(TC)were measured using biochemical methods,HE staining and transmission electron microscopy were used to observe the morphology of liver tissue,PCR was used to detect the mRNA expressions of CD9,CD63,Bax and Bcl-2 in liver tissue,immunohistochemistry and Western blot were used to detect the protein expressions of CD9,CD63,Bax and Bcl-2 in liver tissue.Results Compared with the normal group and the blank group,the contents of FBG,ALT,AST,TG and TC in the model group mice significantly increased(P<0.01);the volume of liver cells increased,the structure was disordered,and a large number of lipid droplets were visible,the cytoplasm showed vacuolar degeneration,mitochondria were severely swollen,and there were a large number of fat particles around lysosomes,the expressions of CD9,CD63,Bax mRNA and protein in liver tissue significantly increased(P<0.01),while the expression of Bcl-2 mRNA and protein significantly decreased(P<0.01).Compared with the model group,the contents of FBG,ALT,AST,TG and TC in the Chinese medicine group mice decreased(P<0.01);the degree of liver cell swelling was reduced,lipid droplets and vacuolar degeneration were significantly improved,and the expressions of CD9,CD63,Bax mRNA and protein in liver tissue were significantly reduced(P<0.01),while the expression of Bcl-2 mRNA and protein significantly increased(P<0.01).Conclusion Zuogui Jiangtang Qingzhi Prescription may inhibit liver cell apoptosis,improve liver injury,and exert therapeutic effects on T2DM combined with NAFLD by reducing the expression of CD9 and CD63,down-regulating Bax,and increasing Bcl-2 expression.
