Effects of Sishen Pills on the Expression of TREK-1 Signaling Pathway in Rats of Irritable Bowel Syndrome with Diarrhea
10.19879/j.cnki.1005-5304.202501040
- VernacularTitle:四神丸对腹泻型肠易激综合征大鼠TREK-1信号通路表达的影响
- Author:
Xiaoyuan LIN
1
;
Shuangyang LUO
;
Na DENG
;
Xuting XIA
;
Jiemin LIU
;
Yujie LI
Author Information
1. 湖南中医药大学第一附属医院,湖南 长沙 410007
- Publication Type:Journal Article
- Keywords:
Sishen Pills;
irritable bowel syndrome with diarrhea;
biportal potassium channel;
TREK-1;
intestinal motility;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(10):94-99
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Sishen Pills on the expression of TREK-1 signaling pathway in rats of irritable bowel syndrome with diarrhea(IBS-D);To explore its mechanism in treating IBS-D.Methods Ten SPF-grade SD rats were randomly selected as the normal group,and the remaining 30 rats were used to prepare the IBS-D rat model by senna gavage combined with water stress avoidance method,and then the modeled rats were randomly divided into the model group,Western medicine group(15.23 mg/kg pivoxyl bromide)and Chinese medicine group(7.32 g/kg Sishen Pills).The medication groups were gavaged by corresponding drugs,and the normal group and model group were given equal volume of pure water,once a day,for consecutive 14 d.The general state of the rats was observed,the contents of serum cyclic adenosine monophosphate(cAMP),protein kinase A(PKA)and phosphorylated extracellular signal-regulated kinase(p-ERK)were detected by ELISA,the positive expressions of biportal potassium channel TREK-1 and p-ERK in colonic tissue were detected by immunofluorescence,the expressions of cAMP,PKA and p-ERK protein were detected by Western blot.Results Compared with the normal group,the body mass of rats in the model group was significantly reduced,and loose stool rate significantly increased(P<0.01);the contents of cAMP,PKA and p-ERK in serum were significantly increased(P<0.05,P<0.01);the positive expression and protein expression of TREK-1 in colonic tissue were significantly reduced,while the positive expression of p-ERK significantly increased(P<0.01),and the expressions of cAMP and PKA proteins significantly increased(P<0.01).Compared with the model group,the body mass of rats in Chinese medince group significantly increased,and loose stool rate significantly decreased(P<0.01);the contents of cAMP,PKA and p-ERK in serum were significantly reduced(P<0.05,P<0.01);the positive expression and protein expression of TREK-1 in colonic tissue significantly increased(P<0.01),while the positive expression of p-ERK significantly decreased(P<0.05),and the expressions of cAMP and PKA proteins significantly decreased(P<0.01).Conclusion Sishen Pills may regulate intestinal motility by affecting the expression of the TREK-1 signaling pathway,thereby treating IBS-D.
