Application value of mycoplasma pneumoniae SAT detection in the diagnosis and treatment of mycoplasma pneumoniae pneumonia in children
10.3969/j.issn.1673-9701.2025.26.008
- VernacularTitle:肺炎支原体SAT检测在儿童肺炎支原体肺炎诊断和治疗中的应用价值
- Author:
Yuanyuan WANG
1
;
Yanmei CHANG
;
Lijuan YU
;
Shuping MENG
Author Information
1. 北京市海淀医院儿科,北京 100081
- Publication Type:Journal Article
- Keywords:
Mycoplasma pneumoniae;
Mycoplasma pneumoniae-antibody;
Simultaneous amplification and testing
- From:
China Modern Doctor
2025;63(26):28-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the application value of mycoplasma pneumonia(MP)real-time fluorescence of RNA simultaneous amplification and testing(SAT)and MP antibody(MP-Ab)in the diagnosis of MP pneumonia(MPP)in children.Methods A total of 242 children with community-acquired pneumonia hospitalized at Beijing Haidian Hospital from September 2023 to October 2024 were enrolled as subjects.The children were divided into MPP group(n=193)and non-MPP group(n=49)based on MPP diagnosis.All children underwent simultaneous MP-SAT testing and initial MP-Ab detection within 24h of admission.MP-SAT results were monitored until they turned negative,with retesting for MP-Ab on 5-7d post-hospitalization in negative cases.The study compared diagnostic accuracy between MP-SAT and MP-Ab methods,while analyzing correlations between MP-SAT negative conversion time and clinical cure duration.Results For children with disease duration ≤ 7 days,MP-SAT demonstrated higher sensitivity than MP-Ab,with statistically significant difference(P＜0.001).The concordance between MP-SAT and initial MP-Ab test results was weak(Kappa=0.072),while the consistency between MP-SAT and follow-up MP-Ab test results was moderate(Kappa=0.614,P＜0.00 1).Both the clinical cure time and SAT seroconversion time were shorter with doxycycline treatment compared to azithromycin therapy.Conclusion The results of MP-SAT can be used to evaluate the condition of MPP children and guide the timely discontinuation of antibiotics.
