Study on the regulatory mechanism of NOD2/RIP2 signaling pathway in inflammatory activation of macrophages in intestinal mucosa
10.3969/j.issn.1673-9701.2025.26.002
- VernacularTitle:NOD2/RIP2信号通路对小肠黏膜中巨噬细胞炎性活化的调控机制
- Author:
Xin WANG
1
;
Tian WU
1
;
Chunbo YANG
1
;
Hongxiang ZHAO
1
;
Xiangyou YU
1
Author Information
1. 新疆医科大学第一附属医院重症医学中心,新疆乌鲁木齐 830011
- Publication Type:Journal Article
- Keywords:
Macrophage;
Inflammatory activation;
Gut-derived systemic inflammation
- From:
China Modern Doctor
2025;63(26):6-8,12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the regulatory mechanism of nucleotide-binding oligomerzation domain 2(NOD2)/receptor interacting protein 2(RIP2)signaling pathway on inflammatory activation of macrophages in intestinal mucosa and provide experimental evidence for intestinal mucosal inflammation caused by bacterial products.Methods Using the THP-1 monocyte cell line,macrophages were stimulated with muramyl dipeptide(MDP)at varying concentrations and durations.mRNA and protein expression levels of NOD2 and RIP2 were detected.The secretion levels of tumor necrosis factor(TNF)-α and interleukin(IL)-1β in the cell culture supernatant were measured.The most effective siRNA targeting RIP2 and optimal transfection concentration were screened,and the impact of RIP2 gene silencing on MDP-induced inflammatory activation of macrophages was observed.Results After silencing the RIP2 gene,MDP induced a significant decrease in TNF-α and IL-1βsecretion in macrophages,but the changes in cell phenotype were not significantly affected.Conclusion This study revealed the important role of NOD2/RIP2 signaling pathway in inflammatory activation of macrophages,and it is possible to effectively inhibit inflammatory activation of macrophages by interfering with this signaling pathway.
