Effects of verbascoside on liver damage and TLR4/NF-κB signaling pathway in D-galactose-induced aging mice
10.3969/j.issn.1001-1528.2025.09.010
- VernacularTitle:毛蕊花糖苷对D-半乳糖诱导衰老小鼠肝脏损伤及TLR4/NF-κB信号通路的影响
- Author:
Chong MA
1
;
Qian-qian WANG
;
Ruo-yu GENG
;
Li-mei WEN
;
Jian-hua YANG
;
Jun-ping HU
Author Information
1. 新疆医科大学药学院,新疆乌鲁木齐 830017
- Publication Type:Journal Article
- Keywords:
verbascoside;
aging;
liver injury;
D-galactose;
TLR4/NF-κB signaling pathway;
inflammation
- From:
Chinese Traditional Patent Medicine
2025;47(9):2888-2893
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the protective effects of verbascoside on D-galactose-induced liver injury in mice and its underlying mechanisms.METHODS C57BL/6J mice were randomly assigned to the normal group,the model group,the vitamin E group(100 mg/kg),and the low-dose and high-dose verbascoside groups(40,80 mg/kg),with 10 mice in each group.Simultaneous administration of medicine and subcutaneous injection of D-galactose(600 mg/kg)went on among the groups except the normal group for 8 weeks.Serum ALT,AST,ALP activities,along with TBil levels were measured using biochemical kits.Hepatic GSH,MDA concentrations,as well as SOD and GSH-Px activities were quantified.Liver pathological morphology was evaluated by HE staining,while hepatic fibrosis area was assessed using Sirius red staining.Western blot analysis determined hepatic expression of IL-6,IL-1β,TNF-ɑ,TLR4,NF-κB p65,IκBɑ and p-IKBɑ proteins.RESULTS Compared to the model group,the groups treated with vitamin E or verbascoside demonstrated significantly reduced body weight(P＜0.05,P＜0.01);increased hepatic index(P＜0.05,P＜0.01);decreased serum activities of ALT,AST and ALP alongsided reduced TBil levels(P＜0.05,P＜0.01);attenuated pathological damage of liver tissue and fibrosis severity;reduced hepatic MDA level(P＜0.05,P＜0.01);and elevated GSH level with enhanced SOD and GSH-Px activities(P＜0.05,P＜0.01).Furthermore,the high-dose verbascoside group showed significantly decreased hepatic expressions of IL-6,IL-1 β,TNF-ɑ,TLR4,NF-κB p65,and p-IKBɑ/IKBɑ proteins(P＜0.05,P＜0.01).CONCLUSION Verbascoside improves D-galactose-induced liver injury through its antioxidant activity,anti-inflammatory effects,and suppression of the TLR4/NF-κB signaling pathway.
