Simultaneous content determination of eleven constituents in Tongxuan Lifei Pills by UPLC and their chemometric investigation
10.3969/j.issn.1001-1528.2025.09.003
- VernacularTitle:UPLC法同时测定通宣理肺丸中11种成分的含量及其化学计量学考察
- Author:
Wan-jun JIN
1
;
Wen-ting HAO
;
Jing LIU
;
Ming-tong ZHANG
;
Lin NI
Author Information
1. 甘肃省药品检验研究院,国家中药材及饮片质量控制重点实验室,甘肃省中藏药检验检测技术工程实验室,甘肃兰州 730030
- Publication Type:Journal Article
- Keywords:
Tongxuan Lifei Pills;
chemical constituents;
content determination;
chemometrics;
UPLC;
cluster analysis;
principal component analysis;
partial least square discriminant analysis
- From:
Chinese Traditional Patent Medicine
2025;47(9):2840-2848
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC method for the simultaneous content determination of liquiritin,ammonium glycyrrhizate,naringin,neohesperidin,hesperidin,rosmarinic acid,baicalin,wogonoside,baicalein,wogonin and praeruptorin A in Tongxuan Lifei Pills,and to make chemometric investigation.METHODS The analysis was performed on a 30 ℃ thermostatic SVEA C18 column(2.1 mm×150 mm,2.5 μm),with the mobile phase comprising of acetonitrile-0.1％phosphoric acid flowing at 0.5 mL/min in a gradient elution manner,and the detection wavelength was set at 250 nm.Subsequently,cluster analysis,principal component analysis and partial least square discriminant analysis were performed.RESULTS Eleven constituents showed good linear relationships within their own ranges(R2＞0.990 0),whose average recoveries were 90.00％-98.32％with the RSDs of 0.35％-1.89％.Forty batches of samples were clustered into 3 types.Baicalein,baicalin,liquiritin,wogonin,wogonoside and neohesperidin were taken as quality differential markers.CONCLUSION This simple and reproducible method can provide the basis for quality control and evaluation of Tongxuan Lifei Pills.
