Simultaneous content determination of thirteen constituents in Zexie Decoction by UPLC-MS/MS
10.3969/j.issn.1001-1528.2025.09.001
- VernacularTitle:UPLC-MS/MS法同时测定泽泻汤中13种成分的含量
- Author:
Pei-qi SUN
1
;
Jia-yang WANG
1
;
Jin-ping WANG
1
;
Yuan-ye JIANG
1
Author Information
1. 上海中医药大学附属普陀医院消化内科,上海 200062
- Publication Type:Journal Article
- Keywords:
Zexie Decoction;
chemical constituents;
content determination;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2025;47(9):2829-2834
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of alisol A,23-acetyl alisol B,alisol A-24-acetate,alisol B,alisol C,23-acetyl alisol C,alisol F,alisol G,atractylenolideⅠ,atractylenolide Ⅱ,atractylenolide Ⅲ,atractylon and ferulic acid in Zexie Decoction.METHODS The analysis was performed on a 30 ℃ thermostatic Waters ACQUITY UPLC HSS C18 column(100 mm×2.1 mm,1.8μm),with the mobile phase comprising of water(containing 0.1％formic acid)-acetonitrile(containing 0.1％formic acid)flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Thirteen constituents showed good linear relationships within their own ranges(r＞0.999 1),whose average recoveries were 95.05％-103.8％with the RSDs of 1.4％-6.7％.CONCLUSION This simple,rapid and accurate method can provide experimental basis for the quality control of Zexie Decoction.
