Methods and advances in molecular detection of Helicobacter pylori resistance
10.3760/cma.j.cn114452-20240725-00405
- VernacularTitle:幽门螺杆菌耐药性的分子检测方法及进展
- Author:
Xin MA
1
;
Chonghui HU
;
Xiuying ZHAO
Author Information
1. 徐州医科大学附属医院消化内科，徐州 221000
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Drug resistance;
Anti-bacterial agents;
Molecular diagnostic techniques;
Polymerase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2025;48(3):419-424
- CountryChina
- Language:Chinese
-
Abstract:
Helicobacter pylori ( H. pylori) is classified as Class I carcinogen in the World Health Organization. It was reported that approximately 90% of gastric cancer cases were associated with H. pylori infection. The eradication of H.plyori can effectively reduce the recurrence rate of peptic ulcers and the incidence of gastric adenocarcinoma. With the escalating antibiotic resistance, the eradication is facing significant challenges. The Maastricht VI/Florence consensus recommends routine antibiotics susceptibility testing to improve the eradication rate of H.plyori. The traditional bacterial culture and identification method require high compliance, laboratory conditions and operative technical, which is difficult to be routinely executed in clinical practice. Molecular biological detection methods have emerged as a proficient tool to identify antibiotic resistance with high efficiency, convenience, accuracy and repeatability. This review summarizes the molecular biological detection strategies for assessing H.plyori resistance, including advantages, limitations, opportunities and challenges.
