Ultrasensitive wash-free quantification of breast cancer-derived small extracellular vesicles via a self-locked DNAzyme nanoprobe
10.3760/cma.j.cn114452-20240607-00299
- VernacularTitle:基于DNAzyme的自锁免洗乳腺癌来源小细胞外囊泡超敏定量检测研究
- Author:
Xiaohui CHEN
1
;
Haixia LIU
;
Ningyu MA
;
Qianqian WU
;
Hengyi CHEN
;
Yi CHEN
;
Wei TU
;
Jinghan CAO
;
Yang LUO
Author Information
1. 重庆大学医学院智慧检验与分子医学中心，重庆400044
- Publication Type:Journal Article
- Keywords:
Extracellular vesicles;
Liquid biopsy;
DNAzyme;
Detection of cancer
- From:
Chinese Journal of Laboratory Medicine
2025;48(3):396-401
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a self-locked DNAzyme nanoprobe-based fluorescence amplification strategy for wash-free and ultrasensitive detection of breast cancer-derived small extracellular vesicles (sEV).Method:A DNAzyme self-locked probe was designed to recognize the epithelial cell adhesion molecule (EpCAM) specifically expressed on breast cancer-derived sEVs. Upon binding to EpCAM, the DNAzyme-lock structure was opened, restoring the DNAzyme cleavage activity. The activated DNAzyme then cyclically cleaved the RNA site on the substrate strand. Fluorescently labeled substrate strands were used to detect sEVs at varying concentrations, and the detection limit and linear range were determined.Results:The DNAzyme self-locked probe successfully identified breast cancer-derived sEVs and generated a fluorescent signal through cyclic cleavage. The proposed method achieved wash-free detection of sEVs, with the fluorescence intensity showing a strong linear correlation with sEV concentration ( R2=0.98). The linear detection range was 1.0×10 2-1.0×1.0 7 particles/μl, with a detection limit of 59 particles/μl. Conclusion:This study established a wash-free and highly sensitive strategy for quantifying breast cancer-derived sEVs, which provides a promising technical approach for the early diagnosis of cancer.
