Exploring the Intervention Effect of Eriocitrin on Intracranial Aneurysm Model Rats Based on SDF-1α/CXCR4 Signaling Path-way
10.16466/j.issn1005-5509.2025.09.001
- VernacularTitle:基于SDF-1α/CXCR4信号通路探究圣草次苷对颅内动脉瘤模型大鼠的干预作用
- Author:
Ke LUO
1
;
Wei HUANG
1
;
Weimin LI
1
Author Information
1. 遂宁市中心医院 四川,遂宁 629018
- Publication Type:Journal Article
- Keywords:
eriocitrin;
intracranial aneurysm;
SDF-1α/CXCR4 signaling pathway;
tumor necrosis factor-α;
interleukin-6;
nitric oxide;
matrix metalloproteinase;
vascular endothelial growth factor
- From:
Journal of Zhejiang Chinese Medical University
2025;49(9):1085-1093
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the intervention effect of eriocitrin on intracranial aneurysm(IA)model rats based on stromal cell derived factor-1α(SDF-1α)/C-X-C motif chemokine receptor 4(CXCR4)signaling pathway.[Methods]IA rat model was established by isolating and ligating the left common carotid artery and renal artery,and removing both ovaries.The rats were randomly assigned into IA group(intragastric and intraperitoneal administration of 0.9%sodium chloride solution),eriocitrin low(intragastric 8 mg·kg-1 of eriocitrin),medium(intragastric 16 mg·kg-1 of eriocitrin)and high(intragastric 32 mg·kg-1 of eriocitrin)dose groups,and eriocitrin high dose(intragastric 32 mg·kg-1)+SDF-1α/CXCR4 pathway activator CTCE-0214(intraperitoneal administration of 10 mg·kg-1 CTCE-0214)group,and the other 10 rats only exposed the left common carotid artery,renal artery and ovaries,which were recorded as sham operation(intragastric and intraperitoneal administration of 0.9%sodium chloride solution)group.Each group was intervened once a day for 8 consecutive weeks.Tweenty-four hours after the last administration,enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and nitric oxide(NO)in each group.Elastica van Gieson staining was used to measure the length of aneurysms,the area of the aneurysm lumen and the length of internal elastic layer rupture.Hematoxylin-eosin(HE)staining was applied to detect pathological changes in arterial aneurysm tissue.Real time-polymerase chain reaction(RT-PCR)was applied to detect the mRNA expression of matrix metalloproteinase-9(MMP-9),vascular endothelial growth factor(VEGF)and MMP-2 in tissues.Western blot was applied to detect the expression of SDF-1α/CXCR4 related proteins in tissues.[Results]Compared with sham operation group,the levels of TNF-α,IL-6;aneurysm length,aneurysm cavity area,internal elastic layer rupture length;MMP-9,VEGF,MMP-2 mRNA expression;SDF-1α and CXCR4 protein expression were prominently higher in IA group,while the level of NO was obviously lower(P<0.05).Compared with IA group,the levels of TNF-α,IL-6;aneurysm length,aneurysm cavity area,internal elastic layer rupture length;MMP-9,VEGF,MMP-2 mRNA expression;SDF-1α and CXCR4 protein expression in eriocitrin low,medium and high dose groups were obviously lower,while the level of NO was prominently higher(P<0.05).Compared with eriocitrin high dose group,the levels of TNF-α,IL-6;aneurysm length,aneurysm cavity area,internal elastic layer rupture length;MMP-9,VEGF,MMP-2 mRNA expression;SDF-1α and CXCR4 protein expression in eriocitrin-high+CTCE-0214 group were prominently higher,while the level of NO was prominently lower(P<0.05).[Conclusion]Eriocitrin alleviates the inflammatory response in IA rats by regulating the SDF-1α/CXCR4 signaling pathway,improves the function of vascular endothelial cells and reduces the risk of aneurysm rupture.
