The effect of ethyl alcohol extract of moutan cortex on virulence factors of Candida tropicalis
10.3760/cma.j.cn114452-20240624-00323
- VernacularTitle:牡丹皮乙醇提取物对热带念珠菌毒力因子的影响
- Author:
Yun TANG
1
;
Xiangren A
;
Peng CHENG
;
Jianwu ZHOU
;
Jideng MA
;
Xiaoya MA
Author Information
1. 青海大学医学院基础医学部，西宁 810016
- Publication Type:Journal Article
- Keywords:
Candida tropicalis;
Ethyl alcohol extract of moutan cortex;
Virulence factors;
Extracellular enzymes;
Biofilms
- From:
Chinese Journal of Laboratory Medicine
2025;48(2):258-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the in vitro bacteriostatic effect of ethyl alcohol extract of Moutan Cortex (EAEMC) on Candida tropicalis and its effect on virulence factors, including aspartic protease, hemolysin, phospholipase, esterase, lipase activities and biofilm. Methods:EAEMC powder was obtained by ultrasonic extraction, decompression concentration and lyophilization; the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EAEMC on 21 clinical strains and one standard strain of Candida tropicalis were determined by microdilution. Five extracellular enzyme activities of Candida tropicalis and the effect of EAEMC on them were detected by the plate assay, and the results were analyzed by ANOVA. The biofilm model of Candida tropicalis was constructed in vitro, and the inhibition rate of EAEMC on Candida tropicalis biofilm was evaluated using the thiazolyl blue (MTT) method. Results:The MIC of EAEMC against Candida tropicalis BNCC335988 was 12.5 g/L and the MBC value was 25 g/L, while for the clinical strains, the MIC was 12.5-25 g/L and the MBC was 25-50 g/L. Aspartic protease, esterase and hemolytic activities of Candida tropicalis were positive, but phospholipase and lipase showed negative activities. At a concentration of 1/2 MIC of EAEMC, the aspartic protease and hemolytic activities of Candida tropicalis were completely inhibited the aspartic protease and hemolytic activities of Candida tropicalis were completely inhibited and the esterase activity was completely inhibited at a concentration of MIC of EAEMC. The inhibition of Candida tropicalis BNCC335988 biofilm by EAEMC reached more than 70% at a concentration of 2MIC, more than 80% at a concentration of 4MIC, and more than 90% at a concentration of 8MIC. Conclusion:EAEMC can achieve bacteriostatic effects by reducing the aspartic protease, esterase and hemolysin activities of Candida tropicalis, as well as inhibiting biofilm formation.
