Discussion on the mechanism of Erchen Pingwei formula treating obesity with insulin resistance based on network pharmacology and molecular docking
10.3969/j.issn.1673-9701.2025.27.010
- VernacularTitle:基于网络药理学和分子对接探讨二陈平胃散治疗伴胰岛素抵抗肥胖症的机制
- Author:
Kai WANG
1
;
Siying WENG
Author Information
1. 浙江中医药大学附属宁波市中医院内分泌科,浙江宁波 315010;浙江中医药大学宁波研究生联合培养基地,浙江宁波 315010
- Publication Type:Journal Article
- Keywords:
Erchen Pingwei formula;
Insulin resistance;
Obesity;
Network pharmacology
- From:
China Modern Doctor
2025;63(27):46-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential mechanism of Erchen Pingwei formula in treating obesity with insulin resistance(IR)by using network pharmacology and molecular docking technology.Methods The main active ingredients and their potential target proteins were obtained from Traditional Chinese Medicine System Pharmacology database and analysis platform and Swiss Target Prediction databases,therapeutic target database,online Mendelian inheritance in man database and genecards databases were used to screen the disease targets related to IR and obesity,and Venny mapping tool was used to screen the intersection of herbal formula and disease targets,and Cytoscape 3.10.0 software was used to construct protein-protein interaction(PPI)network diagrams.Gene Ontology(GO)enrichment,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis on the intersection targets were conducted by using DAVID database,molecular docking and visualization analysis were carried out by Autodock and Pymol.Results 136 active ingredients and 236 active targets of Erchen Pingwei formula were retrieved,with 2527 IR active targets,1598 obesity active targets,and 91 drug-disease intersecting targets.GO and KEGG enrichment analyses showed that quercetin,kaempferol,and naringenin were the main active ingredients of Erchen Pingwei formula,through the regulation of protein kinase B,interleukin-6,tumor protein p53,peroxisome proliferator activated receptor,estrogen receptor α and other core targets,affecting lipid and atherosclerosis,insulin resistance,advanced glycation end product-receptor signaling pathway,and playing a therapeutic role in obesity.Conclusion Erchen Pingwei formula treats obesity with IR via a multi-component,multi-target,and multi-pathway mechanism.
