Establishment of reference intervals for refined immune cell subsets by multi-parameter flow cytometry
10.3760/cma.j.cn114452-20240626-00331
- VernacularTitle:多参数流式细胞术精细化免疫细胞亚群参考区间的建立
- Author:
Chun GU
1
;
Fei WANG
;
Nannan LI
;
Meiqi CHEN
;
Xueyun HOU
;
Jiaxin FEI
;
Runhan MAO
;
Cheng AN
;
Hui WANG
;
Yongzhe LI
;
Guijian LIU
;
Bo PANG
Author Information
1. 中国中医科学院广安门医院检验科，北京100053
- Publication Type:Journal Article
- Keywords:
Flow cytometry;
Immune cells;
Reference interval
- From:
Chinese Journal of Laboratory Medicine
2024;47(12):1411-1418
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The aim of this study is to establish the reference interval of refined immune cell subsets by multi-parameter flow cytometry.Methods:In this cross sectional study, a total of 326 healthy participants were included and divided into two groups based on age: 18-40 years old group and 41-60 years old group. Peripheral venous blood was collected in a fasting status. Flow cytometry tests were performed according to previous consensus article. The analysis of reference interval was conducted according to the documents of Clinical and Laboratory Standards Institute (CLSI) EP28-A3c and Health Industry Standards of the People′s Republic of China WS/T 402-2024.Results:The T,B,NK,DC and monocyte refined immune cell subsets applicable to the reference range of the general population mainly include: CD3 +(56.4%-83.3%),CD4 +TEMRRA (0.2%-11.6%), CD4 +TEM (14.9%-52.8%), CD4 +CD28 +(76.3%-99.9%), CD19 +CD5 +(9.7%-45.8%), CD19 +CD27 -(45.7%-90.0%), CD19 +CD27 +(9.8%-54.0%), CD3 +CD16 +CD56 +(1.3%-20.2%), CD3 -CD19 -CD20 -CD14 -CD56 -HLA-DR +(0.4%-2.0%), Monocyte Mo1 subset CD14 +CD16 -(46.3%-94.6%), monocyte Mo2 subset CD14 +CD16 +(2.8%-49.7%), etc. When the Z-value between different age groups was higher than Z* (3.50 cut-off value), the reference intervals of these subsets should be established independently according to age. Conclusions:In this study, the reference intervals of refined subsets of immune cells by multi-parameter flow cytometry has been preliminarily established. For those subgroups that meet the grouping criteria, age should be fully considered in clinical applications and laboratory validation.
