Analysis of the drug resistence and the whole genome characteristics of mycoplasma pneumoniae strains in Suzhou City from 2023 to 2024
10.3760/cma.j.cn112150-20250107-00016
- VernacularTitle:2023—2024年苏州市肺炎支原体耐药及菌株全基因组特征分析
- Author:
Man YUAN
1
;
Xiaolong WANG
;
Qiang SHEN
;
Xuerong YA
;
Xuan YUAN
;
Ge TIAN
;
Zefeng DONG
Author Information
1. 苏州市疾病预防控制中心病原微生物与生物检验科，苏州 215137
- Publication Type:Journal Article
- Keywords:
Mycoplasma pneumoniae;
Surveillance;
Children;
Resistant mutation;
Whole genome sequencing
- From:
Chinese Journal of Preventive Medicine
2025;59(9):1533-1539
- CountryChina
- Language:Chinese
-
Abstract:
To analyze the prevalence, drug resistance and whole genome characteristics of Mycoplasma pneumoniae (MP) in respiratory throat swab samples of hospitalized children with pneumonia in Suzhou City from 2023 to 2024. Throat swab samples of hospitalized children aged 0-14 years old with pneumonia in Suzhou were collected from September 2023 to September 2024. Real-time fluorenscence quantitative PCR technology was used to detect MP nucleic acid. The results showed that the positive rate of MP in 3 235 samples was 22.44% (726/3 235), with a rate of 55.00% in week 47 of 2023. The positive rate of MP increased with age ( χ2=45.842, P<0.001). The study selected MP nucleic acid test positive samples from week 20 (5.13-5.19) to week 23 (6.3-6.9) of 2024 for isolation, culture and resistance phenotype detection. About 31 MP strains were successfully isolated and cultured, all of which were resistant to macrolides. The next-generation sequencing technology and nanopore sequencing technology were used for genome sequencing. All 31 strains carried the A2063G mutation, with the main prevalent genotype being the P1-1, and the main mlST type being the ST3. Despite the overall genomic similarity between strains being over 99%, there were significant differences between the P1-1 and P1-2 strains in the P1 gene region. In summary, from 2023 to 2024, the main MP type prevalent in Suzhou City is the P1-1 genotype. All isolated MP strains carry an A2063G resistance site mutation and are resistant to macrolides, requiring continuous monitoring and further research.
