Genomic characterization of a case of enterovirus D68 infection in a child from Tongzhou District, Beijing City
10.3760/cma.j.cn112150-20250326-00247
- VernacularTitle:北京市通州区1例儿童肠道病毒D68型毒株的基因组特征分析
- Author:
Bojun ZHEN
1
;
Ping ZHANG
;
Xiaochen GUO
;
Jing ZHANG
;
Yang ZHANG
;
Xiang GAO
;
Fang WANG
;
Jie LI
;
Lin ZOU
Author Information
1. 北京市通州区疾病预防控制中心检验科，北京101100
- Publication Type:Journal Article
- Keywords:
Enterovirus D68;
Capture enrichment;
Whole genome;
Next-generation sequencing
- From:
Chinese Journal of Preventive Medicine
2025;59(7):1108-1112
- CountryChina
- Language:Chinese
-
Abstract:
A throat swab sample from a pediatric case in Tongzhou District, Beijing was identified as enterovirus; the patient was a 1-year-and-8-month-old male sporadic case. Whole-genome sequencing revealed a viral genome length of 7 436 bp. BLAST alignment confirmed the serotype as EV-D68. Phylogenetic analysis of the whole genome indicated that this strain belongs to the B3 clade, showing closer genetic proximity to the 2018 Shanghai strain MW697453 with 99.53% whole-genome nucleotide homology. Genetic and amino acid variation analysis demonstrated that the B3 subclade to which this strain belongs exhibits a nucleotide deletion at positions 718–726, differing from deletion sites observed in other B3 clade strains. A key neuropathogenic amino acid site, T650A, was found to have undergone mutation. Recombination analysis confirmed no cross-clade recombination events in this strain. This study conducted genetic characterization of the strain's evolutionary relationship with EV-D68 strains from different regions and years in China, providing data support for formulating prevention and control measures against EV-D68 infection.
