Isolation of Mycobacterium monacense from lung tissue using mNGS technology: a case report and literature review
10.3760/cma.j.cn114452-20250506-00281
- VernacularTitle:应用mNGS技术从肺组织中分离出慕里黑分枝杆菌1例并文献复习
- Author:
Ze SU
1
;
Qing SUN
;
Dong ZHANG
;
Jie YI
;
Juan DU
;
Qiwen YANG
;
Rui ZHANG
Author Information
1. 中国医学科学院 北京协和医学院 北京协和医院检验科，北京 100730
- Publication Type:Journal Article
- Keywords:
Pneumonia;
Metagenomic next-generation sequencing;
Clinical laboratory technique;
Mycobacterium monacense
- From:
Chinese Journal of Laboratory Medicine
2025;48(11):1461-1464
- CountryChina
- Language:Chinese
-
Abstract:
The patient is a 63-year-old man with a history of chronic illness, presenting with intermittent low-grade fever, recurrent dry cough, and shortness of breath upon exertion. Lung CT showed a nodule in the left lung and a large mass in the right lung with liquefactive necrosis and an air-fluid level accompanied by pleural effusion. Laboratory findings showed significantly increasedf immunoglobulin G (IgG), IgG4, and total immunoglobulin E, along with a mild increase in eosinophils, and moderate elevations in erythrocyte sedimentation rate and high-sensitivity C-reactive protein. Malignant tumors, immunological, and hematological diseases were ruled out. Various antibiotic treatments were ineffective and the condition was recurrent. Conventional methods failed to identify the pathogen. Metagenomic next-generation sequencing (mNGS) technology was used to detect Mycobacterium monacense from lung tissue. Targeted antibiotic treatment was effective with no further episodes of low-grade fever and alleviation of dry cough symptoms. This case underscores the clinical significance of mNGS technology for the diagnosis and treatment of unexplained pulmonary infections, providing a useful reference for clinical diagnostic and treatment.
