Genetic evolution analysis of enteric cytopathogenic human orphan virus type 30 in three cases of infants and young children in Hubei Province
10.3760/cma.j.cn112150-20240131-00103
- VernacularTitle:湖北省3例婴幼儿病例中人肠道致细胞病变孤儿病毒30型的遗传进化分析
- Author:
Kangping ZHOU
1
;
Bing HU
1
;
Kai PAN
1
;
Kun CAI
1
Author Information
1. 湖北省疾病预防控制中心卫生检验检测研究所，武汉 430079
- Publication Type:Journal Article
- Keywords:
Echovirus 30;
Complete genome;
Genetic evolution analysis;
Genetic recombination analysis
- From:
Chinese Journal of Preventive Medicine
2025;59(1):95-100
- CountryChina
- Language:Chinese
-
Abstract:
Three throat swab samples from infants and young children in Hubei Province were identified as positive for enteroviruses. All subjects were one month old, and the onset of symptoms occurred in June; these cases were sporadic and exhibited no clear epidemiological associations. The results of VP1 gene amplification analysis indicated that all three infant cases were attributed to Echovirus 30 (ECHO30). The ECHO30 strain isolated from the three samples was classified as Cluster Ⅲ within the H gene subtype. Whole genome amplification analysis revealed that the ECHO30 strain from Hubei exhibited the highest homology with ECHO30/MW586892/Ashburton/17WQ2027G/USA/2007-08-20 from GenBank, demonstrating nucleotide and amino acid homologies of 97.6% and 99.3%, respectively. Recombination analysis indicated the presence of recombination events in the non-structural protein region of the ECHO30 Hubei strain, with recombinant fragments potentially originating from ECHO30/KY888274/14-397/Germany/2013 and ECHO18 strains Echo18/MN815811/BJ2018-S6363/Beijing/2018. This study provides a comprehensive analysis of the genetic evolution of the ECHO30 strain prevalent in Hubei Province, contributing essential data for the prevention of ECHO30 infections.
