Establishing a dose-response curve for 137Cs γ-ray irradiation-induced chromosomal aberrations in human peripheral blood lymphocytes through automated analysis
10.3760/cma.j.cn112271-20250514-00169
- VernacularTitle:自动识别分析 137Cs γ射线照射诱导人外周血染色体畸变剂量-效应曲线的建立
- Author:
Tianxi ZHANG
1
;
Haipeng YE
1
;
Huijuan YU
1
;
Yan LIU
1
;
Yaping BI
1
;
Yanna LE
1
Author Information
1. 杭州市职业病防治院，杭州 310014
- Publication Type:Journal Article
- Keywords:
Automated analysis;
Chromosomal aberration;
Dose-response curve;
γ-rays;
Unstable chromosome aberration
- From:
Chinese Journal of Radiological Medicine and Protection
2025;45(9):858-862
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a dose-response curve of dicentric chromosomes and centromeric rings (dic+ r) in γ-ray irradiation-induced chromosomal aberrations in human peripheral blood lymphocytes through automated analysis.Methods:Peripheral blood samples from three healthy donors were irradiated in vitro at doses of 0, 0.5, 0.75, 1, 1.5, 2, 3, 4, and 5 Gy and a dose rate of 0.80 Gy/min using a 137Cs γ-ray source. Post-irradiation, lymphocytes were cultured based on standard protocols, harvested using an automatic cell harvester, and prepared on slides using an automatic slide preparation system. dic+ r were analyzed fully automatically using the DCScore software, and a dose-response curve of dic+ r was established through fitting and then validated using the CABAS software. Results:The dose-response curve followed a linear-quadratic model, i. e., y = 0.093 65+ 0.030 21 D+ 0.025 31 D2 ( R2 = 0.999 2), where y was the quantity of dic+ r and D was the absorbed dose of γ-ray irradiation (Gy). Doses to samples for blind validation were estimated using this curve, yielding deviations of less than 24% from the actual irradiation doses. Conclusions:The fully automated analysis of dic+ r in 137Cs γ-ray irradiation-induced chromosomal aberrations, followed by the construction of the dose-response curve, holds significant potential for rapid, high-throughput biodosimetry in large-scale nuclear emergencies.
