Application of a 3D radioactive seed model to glioma cells in mice
10.3760/cma.j.cn112271-20240823-00317
- VernacularTitle:3D放射性粒子模型在小鼠胶质瘤细胞中的应用
- Author:
Xueda LI
1
;
Chuan TIAN
;
Wenfei WANG
;
Peng JIANG
;
Hongtao ZHANG
;
Congxiao WANG
;
Shifeng LIU
;
Xiaokun HU
;
Teng LI
Author Information
1. 青岛大学附属医院介入医学中心，青岛 266000
- Publication Type:Journal Article
- Keywords:
3D printing;
Model;
125I radioactive seed
- From:
Chinese Journal of Radiological Medicine and Protection
2025;45(6):544-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To design a novel model for experiments on in vitro irradiation with radioactive seeds using a treatment planning system (TPS) and 3D printing technology and to preliminarily validate the design scientific rigor of the model via experiments on isodose brachytherapy (BT) and external beam radiotherapy (EBRT) on glioma cells in mice. Methods:The TPS was employed to design the model′s shape and calculate the number and positions of radioactive seeds, and 3D printing technology was utilized to fabricate the experimental model. The GL261 cell line was selected for in vitro irradiation experiments, with the mice divided into the control, EBRT, and BT groups. Mice in the EBRT and BT groups were treated with EBRT and BT, respectively, at doses of 2, 4, and 6 Gy. Then, changes in their cell viability, proliferation, and the level of intracellular reactive oxygen species (ROS) were assessed. Results:The model for in vitro irradiation with radioactive seeds was successfully designed and fabricated. The single photon emission computed tomography (SPECT) verified a uniform radioactive distribution within the model, with no significant cold spots. The BT and EBRT groups displayed decreased cell viability with an increase in the radiation dose. Compared to the EBRT group, the BT group exhibited significantly reduced cell viability (51.33% vs. 22.00%, t = 10.94, P < 0.05) and clone counts (172.67 ± 13.11 vs. 53.67 ± 10.22, t = 8.73, P < 0.05), but a significantly increased level of ROS (102.52 ± 6.87 vs. 144.81 ± 6.01, t = -5.26, P < 0.05) at a dose of 6 Gy. Conclusions:An effective model of in vitro irradiation with radioactive seeds is designed based on TPS and 3D printing technology. This provides an experimental model tool and target for research on the BT and EBRT mechanisms.
