Effect of exogenous short-chain fatty acids preconditioning on expression of zonula occludens-1 in lung tissues of rats undergoing extracorporeal circulation
10.3760/cma.j.cn131073-20241216-01021
- VernacularTitle:外源性短链脂肪酸预处理对体外循环大鼠肺组织ZO-1表达的影响
- Author:
Qi CHU
1
;
Xiaoyan ZHANG
1
;
Huijuan CAO
1
;
Yingjie SUN
1
;
Yugang DIAO
1
;
Tiezheng ZHANG
1
Author Information
1. 北部战区总医院麻醉科，沈阳 110016
- Publication Type:Journal Article
- Keywords:
Fatty acids;
Extracorporeal circulation;
Lung injury;
Zonula occludens-1 protein
- From:
Chinese Journal of Anesthesiology
2025;45(10):1335-1337
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of exogenous short-chain fatty acids (SCFAs) preconditioning on the expression of zonula occludens-1 (ZO-1) in lung tissues of rats undergoing extracorporeal circulation (ECC).Methods:Thirty-six clean-grade healthy adult male Sprague-Dawley rats, weighing 320-420 g, were divided into sham operation group (S group), ECC group (E group) and SCFAs group, with 12 rats in each group. Seven days before the ECC, short-chain fatty acids dissolved in 2 ml of normal saline was given by gavage daily in SCFAs group, while the equal volume of normal saline was given by gavage in S group and E group. On the 8th day, E group and SCFAs group underwent arteriovenous catheterization and ECC for 1 h, while S group only underwent catheterization without ECC. Lung tissues were collected to observe the pathological results and detect the expression of ZO-1 (by Western blot), and the wet/dry lung weight ratio was calculated.Results:Compared with S group, the wet/dry lung weight ratio was significantly increased ( P<0.05), the expression of ZO-1 protein in lung tissue was down-regulated ( P<0.05), and the pathological damage of lung tissues was aggravated in E group and SCFAs group. Compared with E group, the wet/dry lung weight ratio was significantly decreased, the expression of ZO-1 protein in lung tissues was up-regulated ( P<0.05), and the pathological damage of lung tissues was significantly alleviated in SCFAs group. Conclusions:The mechanism by which SCFAs preconditioning attenuates lung injury may be related to up-regulation of ZO-1 expression in lung tissues of rats undergoing ECC.
