Effect of mannitol adenine disodium phosphate preservation solution on exposure of autologous blood salvaged-reinfused red blood cells to phosphatidylserine in dogs
10.3760/cma.j.cn131073-20240726-00413
- VernacularTitle:MAP保存液对犬自体血回收-回输红细胞PS暴露的影响
- Author:
Lei ZHENG
1
;
Daming GOU
Author Information
1. 贵州医科大学附属医院麻醉科，贵阳 550004
- Publication Type:Journal Article
- Keywords:
Mannitol phosphates;
Operative blood salvage;
Erythrocytes;
Phosphatidylserines
- From:
Chinese Journal of Anesthesiology
2025;45(4):454-457
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of mannitol adenine disodium phosphate (MAP) preservation solution on the exposure of autologous blood salvaged-reinfused red blood cells (RBCs) to phosphatidylserine (PS) in dogs.Methods:Twenty healthy mongrel dogs, weighing 10-15 kg, aged 1-3 yr, were divided into 2 groups ( n=9 each) using a random number table method: normal saline group (group NS) and MAP group. The right femoral vein was cannulated for blood-letting (400 ml), and the blood was salvaged using a blood recovery machine. The washing solution for NS group was normal saline, while the washing solution for MAP group was MAP solution. The salvaged RBCs were directly labeled with fluorescein isothiocyanate as a probe and then were re-infused via the left femoral vein over 20-30 min. The ATP content in RBCs was detected by enzyme-linked immunosorbent assay at 30 min before and after blood salvage. Blood samples were collected at 24, 48 and 72 h after the RBCs were re-infused, and the PS exposure rate of cells was detected by flow cytometry. The dogs were sacrificed at 72 h after re-infusion, and the spleen was taken to detect the phagocytic rate of monocytes engulfing the labeled RBCs. Results:Compared with group NS, the ATP content in RBCs was significantly increased after re-infusion of autologous blood, the PS exposure rate of cells was decreased at each time point after re-infusion of autologous blood, and the phagocytic rate of monocytes in the spleen was decreased in group MAP ( P<0.05). Conclusions:MAP preservation solution can reduce the exposure of salvaged-reinfused RBCs to PS, thereby decreasing monocyte phagocytosis and helping to prolong the lifespan of RBCs after re-infusion when used as a washing solution for canine autologous blood salvage.
