The correlation between gut microbiota and inflammatory factors with immune recovery in HIV- infected individuals
10.3760/cma.j.cn112866-20250730-00164
- VernacularTitle:HIV感染者肠道菌群及炎症因子与免疫恢复的相关性分析
- Author:
Xiaoyan GUO
1
;
Hongyan LI
;
Tiantian LI
;
Yanmei JIAO
Author Information
1. 首都医科大学附属北京朝阳医院消化内科，北京 100020
- Publication Type:Journal Article
- Keywords:
Human immunodeficiency virus（HIV）;
Gut microbiota;
Pro-inflammatory factors;
Immune recovery
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(5):565-574
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between gut microbiota characteristics and inflammatory factors with immune recovery in HIV-infected individuals，and to explore the role and clinical significance of gut microbiota in HIV immune recovery.Methods:Sixty HIV-infected individuals and twenty healthy controls（HC）were enrolled. Among them，twenty ones were HIV-infected individuals who had not received antiretroviral therapy（ART），and forty ones were HIV-infected individuals who had received ART for more than two years，including twenty immune responders（IR）and twenty immune non-responders（INR）. Fecal and blood samples were collected from participants. The gut microbiota in fecal samples was analyzed using 16 S rRNA sequencing，while plasma inflammatory factors were detected using Olink proteomics. The correlations between these factors and CD4 + T cell counts，CD4/CD8 ratios，as well as HIV DNA and HIV RNA were analyzed. Results:Compared with the HC group，gut microbiota α diversity in HIV-infected group was reduced，and the microbiota composition changed，with decreased abundance of Firmicutes and related genera，while the abundance of Bacteroidetes and Proteobacteria related genera increased. Compared with the IR group，the INR group showed increased abundance of the Bacteroidetes phylum and decreased abundance of the Firmicutes phylum. LEfSe analysis revealed enrichment of the Flavonifractor genus in the INR group and the Allisonella genus in the IR group. Flavonifractor was positively correlated with HIV DNA and HIV RNA ；Allisonella was positively correlated with CD4? T cell counts and negatively correlated with IL-6，CD8A，and TNF-α expression；and pro-inflammatory factors were positively correlated with the HIV viral reservoir. Conclusions:Reduced gut microbiota diversity and altered composition，as well as increased pro-inflammatory factors，are closely associated with immune recovery and disease progression in HIV-infected individuals.
