Differences in cytokines expression between mild and severe infant cases infected with respiratory syncytial virus
10.3760/cma.j.cn112866-202503-00101
- VernacularTitle:呼吸道合胞病毒感染所致的轻症与重症婴儿的细胞因子表达差异研究
- Author:
Guangyu XUE
1
;
Yuting HU
;
Kexin ZONG
;
Qin LUO
;
Shengnan YANG
;
Miao FENG
;
Xiaoyu YI
;
Zhiqiang XIA
;
Chen GAO
;
Haijun DU
;
Ying LI
;
Ying CHEN
;
Feng HE
;
Yajuan WANG
;
Yingli QU
;
Jin CAO
;
Wenyan TIAN
;
Qinqin SONG
;
Hailan YAO
;
Jun HAN
Author Information
1. 内蒙古科技大学包头医学院公共卫生学院，包头 014040
- Publication Type:Journal Article
- Keywords:
Respiratory syncytial virus infection;
Cytokines;
Infant
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(3):370-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the clinical characteristics and cytokines expression characteristics in infants with mild and severe respiratory syncytial virus (RSV) infection.Methods:From May 2023 to December 2023, plasma samples and clinical information were collected from 16 infants with RSV infection and 14 control infants. Cytek Aurora flow cytometry (Cytek, America) and Enzyme linked immunosorbent assay (ELISA) were used to detect the expression levels of 25 cytokines after mild and severe RSV infection.Results:Cough and nasal obstruction were the main clinical manifestations in infants with mild RSV infection, accompanied by polypnea, wheezing and other symptoms. The main symptoms of severe RSV infection were cough and rales, accompanied by fever and polypnea. In comparison with the control group, the expression levels of IL-2, IL-4, IL-5, IL-6, IL-9, IL-13, IL-22, TNF-α, IFN-α, IFN-β, MIP-1β, I-TAC, ENA-78, GROα, Eotaxin, and MCP-1 in the RSV infection group all exhibited an upregulation trend. Both IP-10 and MIP-3α demonstrated a downward trend in the RSV infection group; however, there was no statistically significant difference ( P>0.05). The levels of IL-10, IFN-γ, MIP-1α, and IL-8 in the RSV infection group were significantly higher than those in the control group, whereas the levels of MIG, TARC, and RANTES in the RSV infection group were significantly lower than those in the control group ( P<0.05). The levels of IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-22, IFN-β, IFN-γ, TNF-α, IL-8, I-TAC, MIP-1β, Eotaxin, and MCP-1 in the mild RSV infection group were significantly higher than those in the severe RSV infection group ( P>0.05). Among these, the levels of MIG, RANTES, TARC, MIP-3α, and ENA-78 in the mild infection group were all lower than those in the severe infection group. The expressions of ENA-78 and MIP-1α in the severe infection group were significantly higher than those in the mild infection group and also higher than those in the control group. There was no significant difference in IP-10 and GROα between the mild and severe RSV infection groups ( P>0.05). Conclusions:The differences in clinical features and cytokines between infants with mild and severe RSV infection provide important data support for the prevention and treatment of RSV infection in infants.
